Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections

Tamás Kustos, Ildikó Kustos, F. Kilár, Gábor Rappai, Béla Kocsis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Despite antibiotic prophylaxis and treatment, the incidence of wound infections in orthopedic surgery is significant. Postoperative wound infection is a multifactorial process, which can be modified by several bacterial factors. Cell surface hydrophobicity of bacteria is a very important physicochemical feature, which has a great influence on the ability of bacteria to adhere to the surface of host cells or medical implants. Methods: In this study, the hydrophobic properties of thirteen bacterial strains (coagulase-negative staphylococci, Staphylococcus aureus and Pseudomonas aeruginosa) isolated from patients with postoperative deep wound infections following orthopedic procedures were determined by the salt aggregation test. Results were compared to the hydrophobicity of three Hungarian standard bacterial strains. The modifying effect of four antibiotics (cefuroxime, cefotaxime, amoxicillin combined with clavulanic acid and amikacin) - applied most often in our Department for prophylaxis and treatment of patients - were analyzed. Results: The cell surface hydrophobicity of certain strains showed considerable changes after antibiotic treatment. These alterations indicated the decrease in hydrophobicity. Supra-inhibitory concentrations (2x minimum inhibitory concentrations, MIC) of the antibiotics were able to induce more frequent alterations in hydrophobicity than sub-inhibitory (0.5x MIC) levels. Conclusions: Alterations in cell surface hydrophobicity caused by antibiotics can modify the adhesion process and thus the pathogenicity of bacterial strains. These changes should be taken into consideration in the management of proper antibiotic prophylaxis and in the treatment of orthopedic patients.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalChemotherapy
Volume49
Issue number5
DOIs
Publication statusPublished - 2003

Fingerprint

Wound Infection
Hydrophobic and Hydrophilic Interactions
Orthopedics
Anti-Bacterial Agents
Bacteria
Surgical Wound Infection
Antibiotic Prophylaxis
Microbial Sensitivity Tests
Orthopedic Procedures
Cefuroxime
Clavulanic Acid
Amikacin
Cefotaxime
Coagulase
Amoxicillin
Therapeutics
Staphylococcus
Pseudomonas aeruginosa
Virulence
Staphylococcus aureus

Keywords

  • Antibiotic treatment
  • Hydrophobicity
  • Orthopedic surgery
  • Wound infections

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections. / Kustos, Tamás; Kustos, Ildikó; Kilár, F.; Rappai, Gábor; Kocsis, Béla.

In: Chemotherapy, Vol. 49, No. 5, 2003, p. 237-242.

Research output: Contribution to journalArticle

Kustos, Tamás ; Kustos, Ildikó ; Kilár, F. ; Rappai, Gábor ; Kocsis, Béla. / Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections. In: Chemotherapy. 2003 ; Vol. 49, No. 5. pp. 237-242.
@article{e8ab638b6ee2471392d87dcd06dde653,
title = "Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections",
abstract = "Background: Despite antibiotic prophylaxis and treatment, the incidence of wound infections in orthopedic surgery is significant. Postoperative wound infection is a multifactorial process, which can be modified by several bacterial factors. Cell surface hydrophobicity of bacteria is a very important physicochemical feature, which has a great influence on the ability of bacteria to adhere to the surface of host cells or medical implants. Methods: In this study, the hydrophobic properties of thirteen bacterial strains (coagulase-negative staphylococci, Staphylococcus aureus and Pseudomonas aeruginosa) isolated from patients with postoperative deep wound infections following orthopedic procedures were determined by the salt aggregation test. Results were compared to the hydrophobicity of three Hungarian standard bacterial strains. The modifying effect of four antibiotics (cefuroxime, cefotaxime, amoxicillin combined with clavulanic acid and amikacin) - applied most often in our Department for prophylaxis and treatment of patients - were analyzed. Results: The cell surface hydrophobicity of certain strains showed considerable changes after antibiotic treatment. These alterations indicated the decrease in hydrophobicity. Supra-inhibitory concentrations (2x minimum inhibitory concentrations, MIC) of the antibiotics were able to induce more frequent alterations in hydrophobicity than sub-inhibitory (0.5x MIC) levels. Conclusions: Alterations in cell surface hydrophobicity caused by antibiotics can modify the adhesion process and thus the pathogenicity of bacterial strains. These changes should be taken into consideration in the management of proper antibiotic prophylaxis and in the treatment of orthopedic patients.",
keywords = "Antibiotic treatment, Hydrophobicity, Orthopedic surgery, Wound infections",
author = "Tam{\'a}s Kustos and Ildik{\'o} Kustos and F. Kil{\'a}r and G{\'a}bor Rappai and B{\'e}la Kocsis",
year = "2003",
doi = "10.1159/000072447",
language = "English",
volume = "49",
pages = "237--242",
journal = "Chemotherapy",
issn = "0009-3157",
publisher = "S. Karger AG",
number = "5",

}

TY - JOUR

T1 - Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections

AU - Kustos, Tamás

AU - Kustos, Ildikó

AU - Kilár, F.

AU - Rappai, Gábor

AU - Kocsis, Béla

PY - 2003

Y1 - 2003

N2 - Background: Despite antibiotic prophylaxis and treatment, the incidence of wound infections in orthopedic surgery is significant. Postoperative wound infection is a multifactorial process, which can be modified by several bacterial factors. Cell surface hydrophobicity of bacteria is a very important physicochemical feature, which has a great influence on the ability of bacteria to adhere to the surface of host cells or medical implants. Methods: In this study, the hydrophobic properties of thirteen bacterial strains (coagulase-negative staphylococci, Staphylococcus aureus and Pseudomonas aeruginosa) isolated from patients with postoperative deep wound infections following orthopedic procedures were determined by the salt aggregation test. Results were compared to the hydrophobicity of three Hungarian standard bacterial strains. The modifying effect of four antibiotics (cefuroxime, cefotaxime, amoxicillin combined with clavulanic acid and amikacin) - applied most often in our Department for prophylaxis and treatment of patients - were analyzed. Results: The cell surface hydrophobicity of certain strains showed considerable changes after antibiotic treatment. These alterations indicated the decrease in hydrophobicity. Supra-inhibitory concentrations (2x minimum inhibitory concentrations, MIC) of the antibiotics were able to induce more frequent alterations in hydrophobicity than sub-inhibitory (0.5x MIC) levels. Conclusions: Alterations in cell surface hydrophobicity caused by antibiotics can modify the adhesion process and thus the pathogenicity of bacterial strains. These changes should be taken into consideration in the management of proper antibiotic prophylaxis and in the treatment of orthopedic patients.

AB - Background: Despite antibiotic prophylaxis and treatment, the incidence of wound infections in orthopedic surgery is significant. Postoperative wound infection is a multifactorial process, which can be modified by several bacterial factors. Cell surface hydrophobicity of bacteria is a very important physicochemical feature, which has a great influence on the ability of bacteria to adhere to the surface of host cells or medical implants. Methods: In this study, the hydrophobic properties of thirteen bacterial strains (coagulase-negative staphylococci, Staphylococcus aureus and Pseudomonas aeruginosa) isolated from patients with postoperative deep wound infections following orthopedic procedures were determined by the salt aggregation test. Results were compared to the hydrophobicity of three Hungarian standard bacterial strains. The modifying effect of four antibiotics (cefuroxime, cefotaxime, amoxicillin combined with clavulanic acid and amikacin) - applied most often in our Department for prophylaxis and treatment of patients - were analyzed. Results: The cell surface hydrophobicity of certain strains showed considerable changes after antibiotic treatment. These alterations indicated the decrease in hydrophobicity. Supra-inhibitory concentrations (2x minimum inhibitory concentrations, MIC) of the antibiotics were able to induce more frequent alterations in hydrophobicity than sub-inhibitory (0.5x MIC) levels. Conclusions: Alterations in cell surface hydrophobicity caused by antibiotics can modify the adhesion process and thus the pathogenicity of bacterial strains. These changes should be taken into consideration in the management of proper antibiotic prophylaxis and in the treatment of orthopedic patients.

KW - Antibiotic treatment

KW - Hydrophobicity

KW - Orthopedic surgery

KW - Wound infections

UR - http://www.scopus.com/inward/record.url?scp=0141839805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141839805&partnerID=8YFLogxK

U2 - 10.1159/000072447

DO - 10.1159/000072447

M3 - Article

VL - 49

SP - 237

EP - 242

JO - Chemotherapy

JF - Chemotherapy

SN - 0009-3157

IS - 5

ER -