Effect of anti-thyroid peroxidase (TPO) antibodies on TPO activity measured by chemiluminescence assay

V. Kaczur, G. Vereb, I. Molnar, G. Krajczar, E. Kiss, N. R. Farid, C. Balázs

Research output: Contribution to journalArticle

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Abstract

A chemiluminescence method was developed to measure thyroid peroxidase (TPO) activity and the inhibitory effect of anti-TPO antibodies in purified porcine TPO. The TPO preparation was characterized kinetically and controlled by Western-blotting technique. The chemiluminescence method proved to be reproducible and much more sensitive than the widely used guaiacol method, being able to detect TPO concentrations of 2.21 x 10-5 g/L vs 6.63 x 10- 2 g/L with the latter. Otherwise, the determinations with the two methods correlated well (r = 0.76). Investigating the effect of IgGs from 23 hypothyroid patients on measured TPO activity, we detected inhibition in 19 cases with the chemiluminescence technique (15 with the guaiacol method). Anti-TPO antibodies showed competitive inhibition of TPO activity with respect to the substrate guaiacol. In both systems, the inhibition is present in the IgG F(ab')2 fragment. We conclude that the high sensitivity of chemiluminescence detection allows routine determination of the inhibition of TPO activity by anti-TPO antibodies.

Original languageEnglish
Pages (from-to)1392-1396
Number of pages5
JournalClinical Chemistry
Volume43
Issue number8
Publication statusPublished - 1997

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Iodide Peroxidase
Chemiluminescence
Luminescence
Assays
Antibodies
Guaiacol
Swine
Immunoglobulin G
Western Blotting

Keywords

  • Autoimmune disease
  • Immunoinhibition
  • Kinetic characterization
  • Thyroiditis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Effect of anti-thyroid peroxidase (TPO) antibodies on TPO activity measured by chemiluminescence assay. / Kaczur, V.; Vereb, G.; Molnar, I.; Krajczar, G.; Kiss, E.; Farid, N. R.; Balázs, C.

In: Clinical Chemistry, Vol. 43, No. 8, 1997, p. 1392-1396.

Research output: Contribution to journalArticle

Kaczur, V, Vereb, G, Molnar, I, Krajczar, G, Kiss, E, Farid, NR & Balázs, C 1997, 'Effect of anti-thyroid peroxidase (TPO) antibodies on TPO activity measured by chemiluminescence assay', Clinical Chemistry, vol. 43, no. 8, pp. 1392-1396.
Kaczur, V. ; Vereb, G. ; Molnar, I. ; Krajczar, G. ; Kiss, E. ; Farid, N. R. ; Balázs, C. / Effect of anti-thyroid peroxidase (TPO) antibodies on TPO activity measured by chemiluminescence assay. In: Clinical Chemistry. 1997 ; Vol. 43, No. 8. pp. 1392-1396.
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AU - Molnar, I.

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AU - Kiss, E.

AU - Farid, N. R.

AU - Balázs, C.

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