Effect of anti-human pan-T monoclonal antibodies on lymphocyte proliferative and cytotoxic functions

Gy Görög, G. Bátory, T. Laskay, G. Petrányi

Research output: Contribution to journalArticle

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Abstract

Effects of anti-human pan-T-specific monoclonal antibodies of the Second International Workshop on Human Leucocyte Differentiation Antigens were investigated in a number of lymphocyte functional tests. Monoclonal antibodies blocking antibody-dependent cytotoxicity (ADCC), PWM-induced IL-2 release, or Con A- and PWM-induced lymphocyte proliferation were found among anti-CD2 and CD3 reagents. Inhibition of lectin-dependent cellular cytotoxicity (LDCC) was found as an exclusive effect of anti-CD2 (the sheep red cell receptor) antibodies. Several anti-CD2s blocked natural killer (NK) activity and/or PWM-induced interferon production. These two effects were exerted by antibodies against epitopes on resting T cells but not by those directed to activation epitopes. The inhibitory activity of individual antibodies in the LDCC and NK tests showed a good correlation. Also, PHA-mediated cytotoxicity (LDCC) and proliferation were in good correlation. Concerning anti-CD3 (T3) reagents, some effects were characteristic for the majority of the antibodies in this group. Namely, induction of proliferation, enhancement of IL-2-dependent cell division, IL-2 consumption by antibody-triggered cells, inhibition of mitogen-induced proliferation but not IL-2 and interferon production were observed. None of the CD3-specific reagents exerted all of these effects. In general, no correlation of the effects with immunoglobulin subclass or CD3 subcluster specificity could be found. Further epitope analysis and affinity data may be required to understand the basis of heterogeneity in functional effects of monoclonal antibodies to the CD3 molecule.

Original languageEnglish
Pages (from-to)184-198
Number of pages15
JournalCellular Immunology
Volume96
Issue number1
DOIs
Publication statusPublished - 1985

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Monoclonal Antibodies
Interleukin-2
Lymphocytes
Lectins
Antibodies
Epitopes
Interferons
CD Antigens
Blocking Antibodies
Lymphocyte Count
Mitogens
Cell Division
Immunoglobulins
Sheep
Cell Proliferation
T-Lymphocytes
Education

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Effect of anti-human pan-T monoclonal antibodies on lymphocyte proliferative and cytotoxic functions. / Görög, Gy; Bátory, G.; Laskay, T.; Petrányi, G.

In: Cellular Immunology, Vol. 96, No. 1, 1985, p. 184-198.

Research output: Contribution to journalArticle

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