Effect of Ala-substitution, N- and C-terminal modification and the presence of counter ions on the structure of amyloid peptide fragment 25-35

Tamás Körtvélyesi, Gergo Kiss, Richard F. Murphy, Botond Penke, Sándor Lovas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of the Ala-substitution, the N- and C-terminal capping and the presence of Ca2+, Mg2+ and Zn2+ on the structure of amyloid peptide fragment, Aβ(25-35), and its analog, [Ala31,32,34,35]Aβ(25-35), was studied. One nanosecond NPT molecular dynamics simulation was used to examine the structural stability of the peptides in water. Calculations were performed using the modified GROMOS-87 force field with SPC/E water model, applying the weak temperature and pressure coupling method. During the simulation of the Aβ(25-35) structure, an initial helical structure was destroyed. In Ac-[Ala31,32,34,35]Aβ(25-35)-NHCH3, however, the helix was stable and even more so in the presence of Ca2+, Mg2+ and Zn2+ cations. Between residues 27 and 34, mainly turn/bend and less frequently helix, respectively, characterized the secondary structure of Aβ(25-35) and [Ala31,32,34,35]Aβ(25-35).

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalJournal of Molecular Structure: THEOCHEM
Volume545
Issue number1-3
DOIs
Publication statusPublished - Jul 9 2001

Keywords

  • Amyloid peptide fragment
  • Aβ(25-35)
  • Molecular dynamics
  • N- and C-capping
  • Secondary structure

ASJC Scopus subject areas

  • Biochemistry
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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