Effect of acidic extracellular pH on the receptor-mediated endocytosis of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages

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Abstract

The effect of acidic extracellular medium on the uptake of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages was studied with the electron microscope. The acidic extracellular medium resulted in acidification of the cytosol in a relatively short period of time as was shown with 5(6) carboxyfluorescein as indicator. At pH 5.5 PAP was internalized. Some of the ligand was found in endosomes; however, a considerable quantity was still present at the cell surface after 5 min internalization. By 30 min the surface was cleared of PAP and the ligand was detectable almost exclusively in endosomes. PAP was never found in lysosomes which were identified by previous loading with cationized ferritin. We conclude that a pH shift of the cytoplasm results in inhibition of endosome-lysosome fusion.

Original languageEnglish
Pages (from-to)529-537
Number of pages9
JournalExperimental Cell Research
Volume184
Issue number2
DOIs
Publication statusPublished - 1989

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Peritoneal Macrophages
Endocytosis
Antigen-Antibody Complex
Peroxidase
Endosomes
Lysosomes
Ligands
Cytosol
Cytoplasm
Electrons

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "Effect of acidic extracellular pH on the receptor-mediated endocytosis of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages",
abstract = "The effect of acidic extracellular medium on the uptake of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages was studied with the electron microscope. The acidic extracellular medium resulted in acidification of the cytosol in a relatively short period of time as was shown with 5(6) carboxyfluorescein as indicator. At pH 5.5 PAP was internalized. Some of the ligand was found in endosomes; however, a considerable quantity was still present at the cell surface after 5 min internalization. By 30 min the surface was cleared of PAP and the ligand was detectable almost exclusively in endosomes. PAP was never found in lysosomes which were identified by previous loading with cationized ferritin. We conclude that a pH shift of the cytoplasm results in inhibition of endosome-lysosome fusion.",
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T1 - Effect of acidic extracellular pH on the receptor-mediated endocytosis of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages

AU - Kiss, A.

AU - Röhlich, P.

PY - 1989

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N2 - The effect of acidic extracellular medium on the uptake of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages was studied with the electron microscope. The acidic extracellular medium resulted in acidification of the cytosol in a relatively short period of time as was shown with 5(6) carboxyfluorescein as indicator. At pH 5.5 PAP was internalized. Some of the ligand was found in endosomes; however, a considerable quantity was still present at the cell surface after 5 min internalization. By 30 min the surface was cleared of PAP and the ligand was detectable almost exclusively in endosomes. PAP was never found in lysosomes which were identified by previous loading with cationized ferritin. We conclude that a pH shift of the cytoplasm results in inhibition of endosome-lysosome fusion.

AB - The effect of acidic extracellular medium on the uptake of peroxidase-antiperoxidase (PAP) immune complex in rat peritoneal macrophages was studied with the electron microscope. The acidic extracellular medium resulted in acidification of the cytosol in a relatively short period of time as was shown with 5(6) carboxyfluorescein as indicator. At pH 5.5 PAP was internalized. Some of the ligand was found in endosomes; however, a considerable quantity was still present at the cell surface after 5 min internalization. By 30 min the surface was cleared of PAP and the ligand was detectable almost exclusively in endosomes. PAP was never found in lysosomes which were identified by previous loading with cationized ferritin. We conclude that a pH shift of the cytoplasm results in inhibition of endosome-lysosome fusion.

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