Effect of a plant-derived natural compound, Flavin7, on the ERK signaling pathway in immortalized mouse proximal tubule cells

Edit Nádasi, István Ember, István Arany

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Abstract

Background: Since MAP kinases represent an important pathway of transducing external stimuli to internal signals in cells, determining their possible role in cancer cells may offer a promising way for the treatment and prognosis of malignant diseases. Our previous experiments have shown that a flavonoid-rich solution, Flavin7, was able to diminish kidney tumor growth in vivo. Materials and Methods: Effects of Flavin7 on the MAPK signaling pathway were determined in immortalized mouse proximal tubule cells by determining cell viability, flow cytometric analysis, luciferase assays and Western blots. Results: At a nontoxic dose, Flavin7 markedly reduced phosphorylation of ERK and inhibited activity of its downstream targets such as Elk1 and CREB via inhibition of the ERK-kinase MEK1. Conclusion: Because of its ability to temporarily inhibit kidney tumor growth and activation of the MEK1/ERK pathway in vitro, further in vivo investigations may determine the potential role of Flavin7 in the treatment of malignancies.

Original languageEnglish
Pages (from-to)871-876
Number of pages6
JournalIn Vivo
Volume21
Issue number5
Publication statusPublished - Sep 1 2007

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Keywords

  • Anticarcinogenic effect
  • Flavin7 extract
  • Mitogen-activated protein kinase pathway
  • Plant-derived natural compounds

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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