Effect of a glutamate receptor antagonist (GYKI 52466) on 4-aminopyridine-induced seizure activity developed in rat cortical slices

Judit Dóczi, Ilona Banczerowski-Pelyhe, Barbara Barna, Ildikó Világi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In the present experiments we have tested the effect of the noncompetitive AMPA antagonist GYKI 52466 (20-80 μM) on spontaneous epileptic discharges developed as the consequence of 4-aminopyridine application in neocortex slices of adult rats. Parallel to the changes of spontaneous activity, the field potentials, evoked by electrical stimulation of the corpus callosum, were also analyzed. Glass microcapillary extracellular recording electrode was positioned in the third layer of the somatosensory cortex slice, while the stimulating electrode was placed at the border of the white and gray matter. 4-aminopyridine and GYKI 52466 were bath-applied. The application of 40 μM GYKI 52466 caused about 40% decrease in the frequency and the amplitude of spontaneous seizures as well as the duration of each discharges developed in 4-aminopyridine. Pre-incubation with the AMPA antagonist effectively inhibited both the development of seizure activity and the maintenance of the discharges. GYKI 52466 also decreased the duration and amplitude of field responses evoked by stimulation of the corpus callosum. This inhibitory effect was dose-dependent. Our data in the in vitro cortex slice epilepsy model suggest that the non-competitive AMPA antagonist GYKI 52466 is a potent anticonvulsant and neuroprotective compound because it reduced the fully developed epileptic discharges or prevented their development. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalBrain Research Bulletin
Volume49
Issue number6
DOIs
Publication statusPublished - Aug 1 1999

Keywords

  • 4-Aminopyridine
  • Brain slice study
  • Epilepsy model
  • GYKI 52466
  • Microelectrophysiology

ASJC Scopus subject areas

  • Neuroscience(all)

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