Effect of 50 Hz magnetic field exposure on the adherent cell contacts of primary mouse Leydig cells in culture

Z. Forgács, Z. Somosy, Csaba Révész, Gábor Jánossy, György Thuróczy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mouse primary testicular intersitital (Leydig) cells obtained from 30-35 g NMRI mice were expose in a CO2 incubator to a sinusoidal 50 Hz/100 μT magnetic field for 48 h. Non-exposed, human choriogonin stimulated (0.1 mlU/ml hCG) cells were also cultured as positive controls. Cells were grown as monolayer on cover slips posited on the bottom of the plastic 24-well culture plates. Following the incubation the cells were fixed and permeabilized with -20°C methanol for 2 hours. For immunocytochemical detection of cadherins, β-catenin and tubulin, cells were incubated over 60 minutes at room temperature with (1:300 diluted) pan-cadherin, anti-β-catenin or antitubulin. Anti-mouse FITC developed in rabbit was used as secondary antibody. Evaluating the samples by fluorescent microscopy, we found that the applied magnetic field exposure increased the amounts of cadherins and β-catenin along the surface of the cell-to-cell contacts. The amount of microtubuli was also elevated and typical shape of cells was changed. The effects of magnetic field exposure were similar to those caused by hCG in the positive controls. Further investigations are required to clarify the subcellular action of applied magnetic field in Leydig cells.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalActa Biologica Szegediensis
Volume47
Issue number1-4
Publication statusPublished - 2003

Fingerprint

primary contact
Leydig cells
Leydig Cells
Magnetic Fields
magnetic fields
Catenins
cell culture
Cadherins
Cell Culture Techniques
Magnetic fields
mice
cadherins
cells
Fluorescein-5-isothiocyanate
Chorionic Gonadotropin
Tubulin
Methanol
Incubators
Monolayers
Microscopic examination

Keywords

  • Cadherin
  • Catenin
  • Immunocytochemistry
  • Leydig cell
  • Magnetic field
  • Tubulin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Neuroscience(all)
  • Applied Microbiology and Biotechnology

Cite this

Effect of 50 Hz magnetic field exposure on the adherent cell contacts of primary mouse Leydig cells in culture. / Forgács, Z.; Somosy, Z.; Révész, Csaba; Jánossy, Gábor; Thuróczy, György.

In: Acta Biologica Szegediensis, Vol. 47, No. 1-4, 2003, p. 27-30.

Research output: Contribution to journalArticle

@article{27333dc851c041049ee4f6237bbd52a0,
title = "Effect of 50 Hz magnetic field exposure on the adherent cell contacts of primary mouse Leydig cells in culture",
abstract = "Mouse primary testicular intersitital (Leydig) cells obtained from 30-35 g NMRI mice were expose in a CO2 incubator to a sinusoidal 50 Hz/100 μT magnetic field for 48 h. Non-exposed, human choriogonin stimulated (0.1 mlU/ml hCG) cells were also cultured as positive controls. Cells were grown as monolayer on cover slips posited on the bottom of the plastic 24-well culture plates. Following the incubation the cells were fixed and permeabilized with -20°C methanol for 2 hours. For immunocytochemical detection of cadherins, β-catenin and tubulin, cells were incubated over 60 minutes at room temperature with (1:300 diluted) pan-cadherin, anti-β-catenin or antitubulin. Anti-mouse FITC developed in rabbit was used as secondary antibody. Evaluating the samples by fluorescent microscopy, we found that the applied magnetic field exposure increased the amounts of cadherins and β-catenin along the surface of the cell-to-cell contacts. The amount of microtubuli was also elevated and typical shape of cells was changed. The effects of magnetic field exposure were similar to those caused by hCG in the positive controls. Further investigations are required to clarify the subcellular action of applied magnetic field in Leydig cells.",
keywords = "Cadherin, Catenin, Immunocytochemistry, Leydig cell, Magnetic field, Tubulin",
author = "Z. Forg{\'a}cs and Z. Somosy and Csaba R{\'e}v{\'e}sz and G{\'a}bor J{\'a}nossy and Gy{\"o}rgy Thur{\'o}czy",
year = "2003",
language = "English",
volume = "47",
pages = "27--30",
journal = "Acta Biologica Szegediensis",
issn = "1588-385X",
publisher = "University of Szeged",
number = "1-4",

}

TY - JOUR

T1 - Effect of 50 Hz magnetic field exposure on the adherent cell contacts of primary mouse Leydig cells in culture

AU - Forgács, Z.

AU - Somosy, Z.

AU - Révész, Csaba

AU - Jánossy, Gábor

AU - Thuróczy, György

PY - 2003

Y1 - 2003

N2 - Mouse primary testicular intersitital (Leydig) cells obtained from 30-35 g NMRI mice were expose in a CO2 incubator to a sinusoidal 50 Hz/100 μT magnetic field for 48 h. Non-exposed, human choriogonin stimulated (0.1 mlU/ml hCG) cells were also cultured as positive controls. Cells were grown as monolayer on cover slips posited on the bottom of the plastic 24-well culture plates. Following the incubation the cells were fixed and permeabilized with -20°C methanol for 2 hours. For immunocytochemical detection of cadherins, β-catenin and tubulin, cells were incubated over 60 minutes at room temperature with (1:300 diluted) pan-cadherin, anti-β-catenin or antitubulin. Anti-mouse FITC developed in rabbit was used as secondary antibody. Evaluating the samples by fluorescent microscopy, we found that the applied magnetic field exposure increased the amounts of cadherins and β-catenin along the surface of the cell-to-cell contacts. The amount of microtubuli was also elevated and typical shape of cells was changed. The effects of magnetic field exposure were similar to those caused by hCG in the positive controls. Further investigations are required to clarify the subcellular action of applied magnetic field in Leydig cells.

AB - Mouse primary testicular intersitital (Leydig) cells obtained from 30-35 g NMRI mice were expose in a CO2 incubator to a sinusoidal 50 Hz/100 μT magnetic field for 48 h. Non-exposed, human choriogonin stimulated (0.1 mlU/ml hCG) cells were also cultured as positive controls. Cells were grown as monolayer on cover slips posited on the bottom of the plastic 24-well culture plates. Following the incubation the cells were fixed and permeabilized with -20°C methanol for 2 hours. For immunocytochemical detection of cadherins, β-catenin and tubulin, cells were incubated over 60 minutes at room temperature with (1:300 diluted) pan-cadherin, anti-β-catenin or antitubulin. Anti-mouse FITC developed in rabbit was used as secondary antibody. Evaluating the samples by fluorescent microscopy, we found that the applied magnetic field exposure increased the amounts of cadherins and β-catenin along the surface of the cell-to-cell contacts. The amount of microtubuli was also elevated and typical shape of cells was changed. The effects of magnetic field exposure were similar to those caused by hCG in the positive controls. Further investigations are required to clarify the subcellular action of applied magnetic field in Leydig cells.

KW - Cadherin

KW - Catenin

KW - Immunocytochemistry

KW - Leydig cell

KW - Magnetic field

KW - Tubulin

UR - http://www.scopus.com/inward/record.url?scp=1342309970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342309970&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 27

EP - 30

JO - Acta Biologica Szegediensis

JF - Acta Biologica Szegediensis

SN - 1588-385X

IS - 1-4

ER -