Effect of 50% human serum on the killing activity of micafungin against eight Candida species using time-kill methodology

Richárd Földi, Judit Szilágyi, G. Kardos, Réka Berényi, Renátó Kovács, László Majoros

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Micafungin activity was determined against 24 wild-type clinical isolates and 5 American Type Culture Collection strains belonging to 8 Candida species in RPMI-1640 with and without 50% serum using broth microdilution and time-kill methodology. MIC values increased from 4- to 128-folds in 50% serum for all Candida species. Micafungin was not fungicidal against C. albicans, C. tropicalis, and against 2 of 3 C. metapsilosis at ≥ 0.25, 1, and 1 μg/mL, respectively, after 48 h with 50% serum, showing good fungistatic activity. Fungicidal activity at ≥ 2, 4, and 32 μg/mL was noticed against C. glabrata, C. inconspicua, and C. krusei isolates, respectively. Micafungin at 8-32 μg/mL showed fungistatic activity against C. parapsilosis and C. orthopsilosis. Serum decreased the in vitro activity of micafungin. With serum binding of echinocandins taken into account, safely fungistatic or fungicidal concentrations seem to require elevated doses against some Candida species, including C. parapsilosis, C. orthopsilosis, and C. krusei.

Original languageEnglish
Pages (from-to)338-342
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume73
Issue number4
DOIs
Publication statusPublished - Aug 2012

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Candida
Serum
Echinocandins
micafungin

Keywords

  • Antifungal susceptibility
  • Echinocandins
  • Impaired killing
  • Serum-based susceptibility testing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Effect of 50% human serum on the killing activity of micafungin against eight Candida species using time-kill methodology. / Földi, Richárd; Szilágyi, Judit; Kardos, G.; Berényi, Réka; Kovács, Renátó; Majoros, László.

In: Diagnostic Microbiology and Infectious Disease, Vol. 73, No. 4, 08.2012, p. 338-342.

Research output: Contribution to journalArticle

Földi, Richárd ; Szilágyi, Judit ; Kardos, G. ; Berényi, Réka ; Kovács, Renátó ; Majoros, László. / Effect of 50% human serum on the killing activity of micafungin against eight Candida species using time-kill methodology. In: Diagnostic Microbiology and Infectious Disease. 2012 ; Vol. 73, No. 4. pp. 338-342.
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