Effect of 4-week feeding of deoxynivalenol- or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.)

Csilla Pelyhe, Benjámin Kövesi, Erika Zándoki, Balázs Kovács, Judit Szabó-Fodor, M. Mézes, K. Balogh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of study was to investigate the effects of T-2 toxin (4.11 mg T-2 toxin and 0.45 mg HT-2 toxin kg−1 feed) and deoxynivalenol (5.96 and 0.33 mg 15-acetyl deoxynivalenol (DON) kg−1 feed) in 1-year-old common carp juveniles in a 4-week feeding trial. The exposure of mycotoxins resulted in increased mortality in both groups consuming mycotoxin-contaminated diet. Parameters of lipid peroxidation were not affected during the trial, and antioxidant defence also did not show response to oxidative stress; however, glutatione peroxidase activity slightly, but significantly, decreased in the T-2 toxin group. Glutathione S-transferase activity showed moderate decrease as effect of T-2 toxin, which suggests its effect on xenobiotic transformation. Reduced glutathione concentration showed moderate changes as effect of DON exposure, but T-2 toxin has no effect. Expression of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes showed different response to mycotoxin exposure. T-2 toxin caused dual response in the expression of gpx4a (early and late downregulation and mid-term upregulation), but continuous upregulation was found as effect of deoxynivalenol. Expression of the other gene, gpx4b, was upregulated by both trichothecenes during the whole period. The results suggested that trichothecenes have some effect on free radical formation and antioxidant defence, but the changes depend on the duration of exposure and the dose applied, and in case of glutathione peroxidase, there was no correlation between expression of genes and enzyme activity.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalMycotoxin Research
Volume32
Issue number2
DOIs
Publication statusPublished - May 1 2016

Fingerprint

T-2 Toxin
Hepatopancreas
Carps
Nutrition
Lipid Peroxidation
Oxidation-Reduction
Glutathione
Diet
Lipids
Mycotoxins
Trichothecenes
Genes
phospholipid-hydroperoxide glutathione peroxidase
Up-Regulation
Antioxidants
Gene Expression
Oxidative stress
Enzyme activity
Xenobiotics
Glutathione Peroxidase

Keywords

  • Common carp
  • Deoxynivalenol
  • Glutathione
  • Lipid peroxidation
  • T-2 toxin

ASJC Scopus subject areas

  • Microbiology
  • Toxicology
  • Biotechnology

Cite this

Effect of 4-week feeding of deoxynivalenol- or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.). / Pelyhe, Csilla; Kövesi, Benjámin; Zándoki, Erika; Kovács, Balázs; Szabó-Fodor, Judit; Mézes, M.; Balogh, K.

In: Mycotoxin Research, Vol. 32, No. 2, 01.05.2016, p. 77-83.

Research output: Contribution to journalArticle

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abstract = "The purpose of study was to investigate the effects of T-2 toxin (4.11 mg T-2 toxin and 0.45 mg HT-2 toxin kg−1 feed) and deoxynivalenol (5.96 and 0.33 mg 15-acetyl deoxynivalenol (DON) kg−1 feed) in 1-year-old common carp juveniles in a 4-week feeding trial. The exposure of mycotoxins resulted in increased mortality in both groups consuming mycotoxin-contaminated diet. Parameters of lipid peroxidation were not affected during the trial, and antioxidant defence also did not show response to oxidative stress; however, glutatione peroxidase activity slightly, but significantly, decreased in the T-2 toxin group. Glutathione S-transferase activity showed moderate decrease as effect of T-2 toxin, which suggests its effect on xenobiotic transformation. Reduced glutathione concentration showed moderate changes as effect of DON exposure, but T-2 toxin has no effect. Expression of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes showed different response to mycotoxin exposure. T-2 toxin caused dual response in the expression of gpx4a (early and late downregulation and mid-term upregulation), but continuous upregulation was found as effect of deoxynivalenol. Expression of the other gene, gpx4b, was upregulated by both trichothecenes during the whole period. The results suggested that trichothecenes have some effect on free radical formation and antioxidant defence, but the changes depend on the duration of exposure and the dose applied, and in case of glutathione peroxidase, there was no correlation between expression of genes and enzyme activity.",
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AU - Pelyhe, Csilla

AU - Kövesi, Benjámin

AU - Zándoki, Erika

AU - Kovács, Balázs

AU - Szabó-Fodor, Judit

AU - Mézes, M.

AU - Balogh, K.

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