Effect of 2,4-dichlorophenol on DPPC/water liposomes studied by X-ray and freeze-fracture electron microscopy

Ágnes Csiszár, Erwin Klumpp, A. Bóta, Krisztián Szegedi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of 2,4-dichlorophenol (DCP) was studied on the fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) - water liposomes. The structure and the thermotropic phase behaviour of the liposomes was examined in the presence of DCP (DCP/DPPC molar ratio, varied from 2×10-2 up to 1) using small- and wide-angle X-ray scattering (SAXS, WAXS) and freeze-fracture electron microscopy. The structural behaviour of the DPPC/DCP/water system was strongly dependent on the concentration of the DCP. In the pretransition range the DCP molecules (at 2×10-2 DCP/DPPC molar ratio) induced the interdigitated phase beside the parent (gel and rippled gel) phases, locally which can be form at higher DCP concentration. When the DCP/DPPC molar ratio was increased the pretransition disappeared and the main transition was shifted to lower temperatures. In the molar ratio range from 2×10-1 up to 5×10-1, a coexistence of different phases was observed in the wide temperature range from 20 up to 40°C. With a further increase of the DCP/DPPC molar ratio (6×10 -1 to 1) only the interdigitated gel phase occurred below 25°C. A schematic phase diagram of DPPC/DCP/water system was constructed to summarise the results.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalChemistry and Physics of Lipids
Volume126
Issue number2
DOIs
Publication statusPublished - Dec 2003

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Liposomes
Electron microscopy
Electron Microscopy
X-Rays
X rays
Water
Gels
Temperature
Schematic diagrams
Phase behavior
colfosceril palmitate
2,4-dichlorophenol
X ray scattering
Phase diagrams
Molecules

Keywords

  • 2,4-Dichlorophenol
  • DPPC liposomes
  • Freeze-fracture
  • Phase diagram
  • SAXS
  • WAXS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Effect of 2,4-dichlorophenol on DPPC/water liposomes studied by X-ray and freeze-fracture electron microscopy. / Csiszár, Ágnes; Klumpp, Erwin; Bóta, A.; Szegedi, Krisztián.

In: Chemistry and Physics of Lipids, Vol. 126, No. 2, 12.2003, p. 155-166.

Research output: Contribution to journalArticle

Csiszár, Ágnes ; Klumpp, Erwin ; Bóta, A. ; Szegedi, Krisztián. / Effect of 2,4-dichlorophenol on DPPC/water liposomes studied by X-ray and freeze-fracture electron microscopy. In: Chemistry and Physics of Lipids. 2003 ; Vol. 126, No. 2. pp. 155-166.
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