Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea

A. Móricz, P. Ott, Tim T. Häbe, András Darcsi, Andrea Böszörményi, Ágnes Alberti, Dániel Krüzselyi, P. Csontos, S. Béni, Gertrud E. Morlock

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A nontargeted, effect-directed screening (bioprofiling) and a subsequent highly targeted characterization of antibacterial compounds from plant matrices is demonstrated on the example of Solidago virgaurea root extracts. The procedure comprises high-performance thin-layer chromatography (HPTLC) coupled with six bacterial bioassays including two plant pathogens, a radical scavenging assay, an acetylcholinesterase assay as well as in situ and ex situ mass spectrometric analyses. In situ mass spectra were directly recorded from the adsorbent using the Direct Analysis in Real Time interface (HPTLC-DART-MS), whereas ex situ mass spectra were recorded using an elution head-based interface (HPTLC-ESI-MS). For further bioassay-guided isolation of the main antimicrobial compounds, flash chromatographic fractionation and semipreparative high-performance liquid chromatographic purification were used and nuclear magnetic resonance data allowed the identification of the unknown antimicrobial compounds as 2Z,8Z- and 2E,8Z-matricaria esters. The discovered antibacterial activity was confirmed and specified by a luminometric assay and as minimal inhibitory concentration in the liquid phase.

Original languageEnglish
Pages (from-to)8202-8209
Number of pages8
JournalAnalytical Chemistry
Volume88
Issue number16
DOIs
Publication statusPublished - Aug 16 2016

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Thin layer chromatography
Assays
Bioassay
Scavenging
Liquids
Pathogens
Acetylcholinesterase
Fractionation
Adsorbents
Purification
Screening
Esters
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea. / Móricz, A.; Ott, P.; Häbe, Tim T.; Darcsi, András; Böszörményi, Andrea; Alberti, Ágnes; Krüzselyi, Dániel; Csontos, P.; Béni, S.; Morlock, Gertrud E.

In: Analytical Chemistry, Vol. 88, No. 16, 16.08.2016, p. 8202-8209.

Research output: Contribution to journalArticle

Móricz, A. ; Ott, P. ; Häbe, Tim T. ; Darcsi, András ; Böszörményi, Andrea ; Alberti, Ágnes ; Krüzselyi, Dániel ; Csontos, P. ; Béni, S. ; Morlock, Gertrud E. / Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea. In: Analytical Chemistry. 2016 ; Vol. 88, No. 16. pp. 8202-8209.
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