Eel sperm cryopreservation: An overview

Juan German Herranz-Jusdado, Victor Gallego, Marina Morini, Christoffer Rozenfeld, Luz Pérez, Tamás Müller, A. Horváth, Hiromi Ohta, Juan F. Asturiano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The eels are teleost fishes from the order Anguilliformes that includes several species with high commercial value. Due to the high interest for aquaculture production of some eel species and for the need to restore eel species that are endangered, several research groups have directed their research toward developing protocols to cryopreserve the spermatozoa of Japanese eel (Anguilla japonica) and European eel (Anguilla anguilla). In this review, we provide an overview on the different protocols that have been developed so far. The first developed protocols used DMSO as cryoprotectant in both species with good success, obtaining sperm motilities of over 45% in Japanese eel and over 35% in European eel. Moreover, sperm cryopreserved using DMSO was successfully used in fertilization trials, although with low fertilization rates. However, recent studies show that DMSO produce epigenetic changes in eel sperm and therefore, the last developed protocols used methanol as cryoprotectant instead. Cryopreservation protocols using methanol as cryoprotectant, showed improved motility values in both Japanese and European eel. In addition, the latest protocols have been adapted to cryopreserve larger volumes of sperm of up to 5 mL, which is useful for larger scale fertilization trials. The present study introduces the state of the art and future perspectives of the eel sperm cryopreservation to be applied in aquaculture and biological conservation programs.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalTheriogenology
Volume133
DOIs
Publication statusPublished - Jul 15 2019

Fingerprint

Eels
Cryopreservation
eel
Anguilla japonica
cryopreservation
Anguilla anguilla
Spermatozoa
spermatozoa
cryoprotectants
fertilization (reproduction)
Anguilla
aquaculture
methanol
Anguilliformes
Dimethyl Sulfoxide
Fertilization
Aquaculture
conservation programs
sperm motility
epigenetics

Keywords

  • Anguilla
  • DMSO
  • Fish
  • Methanol
  • Spermatozoa motility
  • Vitrification

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Herranz-Jusdado, J. G., Gallego, V., Morini, M., Rozenfeld, C., Pérez, L., Müller, T., ... Asturiano, J. F. (2019). Eel sperm cryopreservation: An overview. Theriogenology, 133, 210-215. https://doi.org/10.1016/j.theriogenology.2019.03.033

Eel sperm cryopreservation : An overview. / Herranz-Jusdado, Juan German; Gallego, Victor; Morini, Marina; Rozenfeld, Christoffer; Pérez, Luz; Müller, Tamás; Horváth, A.; Ohta, Hiromi; Asturiano, Juan F.

In: Theriogenology, Vol. 133, 15.07.2019, p. 210-215.

Research output: Contribution to journalArticle

Herranz-Jusdado, JG, Gallego, V, Morini, M, Rozenfeld, C, Pérez, L, Müller, T, Horváth, A, Ohta, H & Asturiano, JF 2019, 'Eel sperm cryopreservation: An overview', Theriogenology, vol. 133, pp. 210-215. https://doi.org/10.1016/j.theriogenology.2019.03.033
Herranz-Jusdado JG, Gallego V, Morini M, Rozenfeld C, Pérez L, Müller T et al. Eel sperm cryopreservation: An overview. Theriogenology. 2019 Jul 15;133:210-215. https://doi.org/10.1016/j.theriogenology.2019.03.033
Herranz-Jusdado, Juan German ; Gallego, Victor ; Morini, Marina ; Rozenfeld, Christoffer ; Pérez, Luz ; Müller, Tamás ; Horváth, A. ; Ohta, Hiromi ; Asturiano, Juan F. / Eel sperm cryopreservation : An overview. In: Theriogenology. 2019 ; Vol. 133. pp. 210-215.
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