Economic approach to robotic sample pretreatment in high-performance liquid chromatography

Antal Tolokán, Lajos Gödörházy, Viola Horváth, Andrea Hrabéczy-Páll, Zsuzsanna Niegreisz, Emilia Kocsi, George Horvai, Imre Klebovich, Katalin Balogh-Nemes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Economic automation of multistep analytical sample preparation is still a largely unsolved problem. Existing systems either handle only a few steps of a complex analytical scheme or they offer a complete solution at relatively high cost. This paper shows, using a rather general example that it is possible to construct a system in the analytical laboratory with an optimal balance between automating most steps of the analysis scheme and still keeping the costs of the system within bounds. A practical example from pharmacokinetic analysis is presented; pretreatment of blood plasma samples for nifedipine determination by high-performance liquid chromatography. The automated method involves steps such as aliquoting, pH adjustment, liquid-liquid extraction and evaporation to dryness. The system is built around a robotic arm from commercial equipment not designed originally for use with a robot. Analytical results and costs are compared to the manual method.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalJournal of Chromatography A
Volume771
Issue number1-2
DOIs
Publication statusPublished - May 30 1997

Keywords

  • Automation
  • Nifedipine
  • Sample handling

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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