EBP1 regulates organ size through cell growth and proliferation in plants

Beatrix M. Horváth, Z. Magyar, Yuexing Zhang, Anne W. Hamburger, László Bakó, Richard G F Visser, Christian W B Bachem, László Bögre

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Plant organ size shows remarkable uniformity within species indicating strong endogenous control. We have identified a plant growth regulatory gene, functionally and structurally homologous to human EBP1. Plant EBP1 levels are tightly regulated; gene expression is highest in developing organs and correlates with genes involved in ribosome biogenesis and function. EBP1 protein is stabilised by auxin. Elevating or decreasing EBP1 levels in transgenic plants results in a dose-dependent increase or reduction in organ growth, respectively. During early stages of organ development, EBP1 promotes cell proliferation, influences cell-size threshold for division and shortens the period of meristematic activity. In postmitotic cells, it enhances cell expansion. EBP1 is required for expression of cell cycle genes; CyclinD3;1, ribonucleotide reductase 2 and the cyclin-dependent kinase B1;1. The regulation of these genes by EBP1 is dose and auxin dependent and might rely on the effect of EBP1 to reduce RBR1 protein level. We argue that EBP1 is a conserved, dose-dependent regulator of cell growth that is connected to meristematic competence and cell proliferation via regulation of RBR1 level.

Original languageEnglish
Pages (from-to)4909-4920
Number of pages12
JournalEMBO Journal
Volume25
Issue number20
DOIs
Publication statusPublished - Oct 18 2006

Fingerprint

Organ Size
Cell proliferation
Cell growth
Indoleacetic Acids
Genes
Cell Proliferation
Growth
CDC2 Protein Kinase
Ribonucleotide Reductases
cdc Genes
Genetically Modified Plants
Cyclin-Dependent Kinase 2
Regulator Genes
Ribosomes
Cell Size
Mental Competency
Proteins
Gene expression
Gene Expression
Cells

Keywords

  • Cell growth
  • Cell proliferation
  • EBP1
  • Organ growth
  • Ribosome biogenesis

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Horváth, B. M., Magyar, Z., Zhang, Y., Hamburger, A. W., Bakó, L., Visser, R. G. F., ... Bögre, L. (2006). EBP1 regulates organ size through cell growth and proliferation in plants. EMBO Journal, 25(20), 4909-4920. https://doi.org/10.1038/sj.emboj.7601362

EBP1 regulates organ size through cell growth and proliferation in plants. / Horváth, Beatrix M.; Magyar, Z.; Zhang, Yuexing; Hamburger, Anne W.; Bakó, László; Visser, Richard G F; Bachem, Christian W B; Bögre, László.

In: EMBO Journal, Vol. 25, No. 20, 18.10.2006, p. 4909-4920.

Research output: Contribution to journalArticle

Horváth, BM, Magyar, Z, Zhang, Y, Hamburger, AW, Bakó, L, Visser, RGF, Bachem, CWB & Bögre, L 2006, 'EBP1 regulates organ size through cell growth and proliferation in plants', EMBO Journal, vol. 25, no. 20, pp. 4909-4920. https://doi.org/10.1038/sj.emboj.7601362
Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RGF et al. EBP1 regulates organ size through cell growth and proliferation in plants. EMBO Journal. 2006 Oct 18;25(20):4909-4920. https://doi.org/10.1038/sj.emboj.7601362
Horváth, Beatrix M. ; Magyar, Z. ; Zhang, Yuexing ; Hamburger, Anne W. ; Bakó, László ; Visser, Richard G F ; Bachem, Christian W B ; Bögre, László. / EBP1 regulates organ size through cell growth and proliferation in plants. In: EMBO Journal. 2006 ; Vol. 25, No. 20. pp. 4909-4920.
@article{b6a10d14f47b46209d9ed2a2461e2dc0,
title = "EBP1 regulates organ size through cell growth and proliferation in plants",
abstract = "Plant organ size shows remarkable uniformity within species indicating strong endogenous control. We have identified a plant growth regulatory gene, functionally and structurally homologous to human EBP1. Plant EBP1 levels are tightly regulated; gene expression is highest in developing organs and correlates with genes involved in ribosome biogenesis and function. EBP1 protein is stabilised by auxin. Elevating or decreasing EBP1 levels in transgenic plants results in a dose-dependent increase or reduction in organ growth, respectively. During early stages of organ development, EBP1 promotes cell proliferation, influences cell-size threshold for division and shortens the period of meristematic activity. In postmitotic cells, it enhances cell expansion. EBP1 is required for expression of cell cycle genes; CyclinD3;1, ribonucleotide reductase 2 and the cyclin-dependent kinase B1;1. The regulation of these genes by EBP1 is dose and auxin dependent and might rely on the effect of EBP1 to reduce RBR1 protein level. We argue that EBP1 is a conserved, dose-dependent regulator of cell growth that is connected to meristematic competence and cell proliferation via regulation of RBR1 level.",
keywords = "Cell growth, Cell proliferation, EBP1, Organ growth, Ribosome biogenesis",
author = "Horv{\'a}th, {Beatrix M.} and Z. Magyar and Yuexing Zhang and Hamburger, {Anne W.} and L{\'a}szl{\'o} Bak{\'o} and Visser, {Richard G F} and Bachem, {Christian W B} and L{\'a}szl{\'o} B{\"o}gre",
year = "2006",
month = "10",
day = "18",
doi = "10.1038/sj.emboj.7601362",
language = "English",
volume = "25",
pages = "4909--4920",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "20",

}

TY - JOUR

T1 - EBP1 regulates organ size through cell growth and proliferation in plants

AU - Horváth, Beatrix M.

AU - Magyar, Z.

AU - Zhang, Yuexing

AU - Hamburger, Anne W.

AU - Bakó, László

AU - Visser, Richard G F

AU - Bachem, Christian W B

AU - Bögre, László

PY - 2006/10/18

Y1 - 2006/10/18

N2 - Plant organ size shows remarkable uniformity within species indicating strong endogenous control. We have identified a plant growth regulatory gene, functionally and structurally homologous to human EBP1. Plant EBP1 levels are tightly regulated; gene expression is highest in developing organs and correlates with genes involved in ribosome biogenesis and function. EBP1 protein is stabilised by auxin. Elevating or decreasing EBP1 levels in transgenic plants results in a dose-dependent increase or reduction in organ growth, respectively. During early stages of organ development, EBP1 promotes cell proliferation, influences cell-size threshold for division and shortens the period of meristematic activity. In postmitotic cells, it enhances cell expansion. EBP1 is required for expression of cell cycle genes; CyclinD3;1, ribonucleotide reductase 2 and the cyclin-dependent kinase B1;1. The regulation of these genes by EBP1 is dose and auxin dependent and might rely on the effect of EBP1 to reduce RBR1 protein level. We argue that EBP1 is a conserved, dose-dependent regulator of cell growth that is connected to meristematic competence and cell proliferation via regulation of RBR1 level.

AB - Plant organ size shows remarkable uniformity within species indicating strong endogenous control. We have identified a plant growth regulatory gene, functionally and structurally homologous to human EBP1. Plant EBP1 levels are tightly regulated; gene expression is highest in developing organs and correlates with genes involved in ribosome biogenesis and function. EBP1 protein is stabilised by auxin. Elevating or decreasing EBP1 levels in transgenic plants results in a dose-dependent increase or reduction in organ growth, respectively. During early stages of organ development, EBP1 promotes cell proliferation, influences cell-size threshold for division and shortens the period of meristematic activity. In postmitotic cells, it enhances cell expansion. EBP1 is required for expression of cell cycle genes; CyclinD3;1, ribonucleotide reductase 2 and the cyclin-dependent kinase B1;1. The regulation of these genes by EBP1 is dose and auxin dependent and might rely on the effect of EBP1 to reduce RBR1 protein level. We argue that EBP1 is a conserved, dose-dependent regulator of cell growth that is connected to meristematic competence and cell proliferation via regulation of RBR1 level.

KW - Cell growth

KW - Cell proliferation

KW - EBP1

KW - Organ growth

KW - Ribosome biogenesis

UR - http://www.scopus.com/inward/record.url?scp=33750219419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750219419&partnerID=8YFLogxK

U2 - 10.1038/sj.emboj.7601362

DO - 10.1038/sj.emboj.7601362

M3 - Article

VL - 25

SP - 4909

EP - 4920

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 20

ER -