EBER oligonucleotide RNA in situ hybridization in EBV associated neoplasms

T. Tornóczky, Gábor Kelényi, László Pajor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In virus associated diseases identification of viruses in cells can contribute to the understanding of the pathogenesis and may also help to establish the diagnosis. In the present communication, the effects of the microwave pretreatment (MWP) and that of the proteinase-K enzymatic predigestion (PKD) on EBER RNA oligonucleotide in situ hybridization (EBER- RNA-ISH) (EBER: Epstein-Barr-Encoded-(Early)-RNA) were studied. The efficacy of two EBV detecting methods, latent membrane protein-1 (LMP-1) immunohistochemistry and EBER-RNA-ISH were also compared. Our results show that microwave pretreatment enhances the intensity of the ISH signals and preserves significantly better the structure of the tissues compared with enzymatic predigestion. EBER-RNA-ISH, mainly in the nasopharyngeal carcinoma cases, showed a more frequent positivity than the immunohistochemical reaction for LMP-1, however in case of the Warthin's tumor only the LMP-1 protein was expressed.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalPathology and Oncology Research
Volume4
Issue number3
Publication statusPublished - 1998

Fingerprint

Human Herpesvirus 4
Oligonucleotides
In Situ Hybridization
RNA
Membrane Proteins
Microwaves
Neoplasms
Adenolymphoma
Endopeptidase K
Virus Diseases
Immunohistochemistry
Viruses
Proteins

Keywords

  • EBER
  • EBV
  • LMP-1
  • Lymphoma
  • Microwave
  • Nasopharyngeal carcinoma
  • Warthin's tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

EBER oligonucleotide RNA in situ hybridization in EBV associated neoplasms. / Tornóczky, T.; Kelényi, Gábor; Pajor, László.

In: Pathology and Oncology Research, Vol. 4, No. 3, 1998, p. 201-205.

Research output: Contribution to journalArticle

Tornóczky, T. ; Kelényi, Gábor ; Pajor, László. / EBER oligonucleotide RNA in situ hybridization in EBV associated neoplasms. In: Pathology and Oncology Research. 1998 ; Vol. 4, No. 3. pp. 201-205.
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