EB virus induction is associated with B-cell maturation

D. H. Crawford, I. Ando

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

EB virus genome-carrying B-cell lines have been double-stained for B-cell activation or maturation antigens and viral antigens using indirect immunofluorescence. The vast majority of cells express activation antigens, whereas the plasma cell antigen PC1 is present on only a few cells. Staining for this antigen doubles with staining for EB viral capsid antigen. Thus it marks cells in the virus replicative cycle, and these cells are negative for the EB virus nuclear antigen EBNA. We suggest that within EB virus genome-carrying B-cell lines the maturation of a few cells to the stage of expression of plasma-cell markers leads to the loss of EBNA expression and consequent loss of the immortalized state of the cell. This would allow completion of the lytic cycle with the production of virus particles and cell death. The implications of these findings are discussed.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalImmunology
Volume59
Issue number3
Publication statusPublished - 1986

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Virus Activation
B-Lymphocytes
Viruses
Viral Antigens
Plasma Cells
Antigens
B-Cell Maturation Antigen
CD80 Antigens
Genome
Staining and Labeling
Cell Line
Nuclear Antigens
Capsid
Indirect Fluorescent Antibody Technique
Virion
Cell Cycle
Cell Death

ASJC Scopus subject areas

  • Immunology

Cite this

EB virus induction is associated with B-cell maturation. / Crawford, D. H.; Ando, I.

In: Immunology, Vol. 59, No. 3, 1986, p. 405-409.

Research output: Contribution to journalArticle

Crawford, DH & Ando, I 1986, 'EB virus induction is associated with B-cell maturation', Immunology, vol. 59, no. 3, pp. 405-409.
Crawford, D. H. ; Ando, I. / EB virus induction is associated with B-cell maturation. In: Immunology. 1986 ; Vol. 59, No. 3. pp. 405-409.
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