Early specific free radical-related cytotoxicity of gas phase cigarette smoke and its paradoxical temporary inhibition by tar. An electron paramagnetic resonance study with the spin trap DEPMPO

Marcel Culcasi, Agnès Muller, Anne Mercier, Jean Louis Clément, Olivier Payet, A. Rockenbauer, Véronique Marchand, Sylvia Pietri

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Electron paramagnetic resonance (EPR) spin trapping studies demonstrated aqueous tar particulate matter (TPM) and gas phase cigarette smoke (GPCS) to behave as different sources of free radicals in cigarette smoke (CS) but their cytotoxic implications have been only assessed in CS due to its relevance to the natural smoking process. Using a sensitive spin trapping detection with 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), this study compared the respective roles of CS- and GPCS-derived free radicals on smoke-induced cytotoxicity and lipid peroxidation of filtered and unfiltered, machine-smoked experimental and reference cigarettes yielding a wide range of TPM yields. In buffer bubbled with CS the DEPMPO/superoxide spin adduct was the major detected nitroxide. Use of appropriate control experiments with nitric oxide radical (NO{radical dot}) or carbonyl sulfide, and a computer analysis of spin adduct diastereoisomery showed that the hydroxyl radical (HO{radical dot}) adduct of DEPMPO seen in GPCS-bubbled was rather related to metal-catalyzed nucleophilic synthesis than to direct HO{radical dot} trapping. Unexpectedly a protective effect of TPM on murine 3T3 fibroblasts was observed in early (

Original languageEnglish
Pages (from-to)215-231
Number of pages17
JournalChemico-Biological Interactions
Volume164
Issue number3
DOIs
Publication statusPublished - Dec 15 2006

Fingerprint

Tars
Electron Spin Resonance Spectroscopy
Cytotoxicity
Smoke
Tobacco Products
Oxides
Free Radicals
Paramagnetic resonance
Gases
Particulate Matter
Spin Trapping
pyrroline
Inhibition (Psychology)
Fibroblasts
Superoxides
Hydroxyl Radical
Lipid Peroxidation
Buffers
Nitric Oxide
Smoking

Keywords

  • Cytotoxicity
  • DEPMPO
  • Electron paramagnetic resonance
  • Gas phase cigarette smoke
  • Lipid peroxidation
  • Spin trapping

ASJC Scopus subject areas

  • Toxicology

Cite this

Early specific free radical-related cytotoxicity of gas phase cigarette smoke and its paradoxical temporary inhibition by tar. An electron paramagnetic resonance study with the spin trap DEPMPO. / Culcasi, Marcel; Muller, Agnès; Mercier, Anne; Clément, Jean Louis; Payet, Olivier; Rockenbauer, A.; Marchand, Véronique; Pietri, Sylvia.

In: Chemico-Biological Interactions, Vol. 164, No. 3, 15.12.2006, p. 215-231.

Research output: Contribution to journalArticle

Culcasi, Marcel ; Muller, Agnès ; Mercier, Anne ; Clément, Jean Louis ; Payet, Olivier ; Rockenbauer, A. ; Marchand, Véronique ; Pietri, Sylvia. / Early specific free radical-related cytotoxicity of gas phase cigarette smoke and its paradoxical temporary inhibition by tar. An electron paramagnetic resonance study with the spin trap DEPMPO. In: Chemico-Biological Interactions. 2006 ; Vol. 164, No. 3. pp. 215-231.
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