Early replication signals in nuclei of Chinese hamster ovary cells

G. Banfalvi, H. Tanke, A. K. Raap, J. Slats, M. van der Ploeg

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7 Citations (Scopus)

Abstract

DNA replication sites generally known as replicon domains were resolved as individual replication signals in interphase nuclei of permeabilized Chinese hamster ovary cells by immunofluorescent microscopy. Biotin-11-dUTP was utilized as a tool to label newly replicated DNA in permeable cells and to study the distribution of nascent DNA in pulselabel and in pulsechase experiments. Active sites of DNA replication were visualized in exponentially growing cells and in synchronized cultures throughout the S phase. Fluorescent images of replication sites were analyzed by standard fluorescense microscopy and in three dimensions by confocal laser scanning microscopy. The rapid increase in number of discrete foci of newly replicated DNA is an indication that DNA synthesis starts at limited number of sites in mammalian nuclei rather than at thousands of foci at the same time.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalHistochemistry
Volume94
Issue number4
DOIs
Publication statusPublished - Aug 1 1990

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences(all)

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    Banfalvi, G., Tanke, H., Raap, A. K., Slats, J., & van der Ploeg, M. (1990). Early replication signals in nuclei of Chinese hamster ovary cells. Histochemistry, 94(4), 435-440. https://doi.org/10.1007/BF00266452