Early nuclear antigen as DNA-binding protein in cytomegalovirus-infected cells

L. Gergely, J. Czegledy, L. Vaczi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Early nuclear complement-fixing antigen in cytomegalovirus (CMV) (strain Ad 169)-infected human embryonic fibroblasts is described. The nuclear antigen was solubilized from CMV-infected cells by high salt treatment. DNA-binding properties were studied by DNA-cellulose chromatography. The purified antigen, eluted from double-stranded DNA-cellulose columns, was added to acid-fixed nuclear preparations from human embryonic fibroblasts and the exposed to human sera containing antibodies to CMV. Positive staining was obtained by anti-complement immunofluorescence. These data identify the CMV-determined nuclear antigen as a DNA-binding protein. In this respect, it is similar to the Epstein-Barr virus nuclear antigen.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalIntervirology
Volume13
Issue number6
Publication statusPublished - 1980

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Nuclear Antigens
DNA-Binding Proteins
Cytomegalovirus
Fibroblasts
Epstein-Barr Virus Nuclear Antigens
Antigens
DNA
Fluorescent Antibody Technique
Chromatography
Salts
Staining and Labeling
Acids
Antibodies
Serum
DNA-cellulose
Therapeutics

ASJC Scopus subject areas

  • Virology

Cite this

Early nuclear antigen as DNA-binding protein in cytomegalovirus-infected cells. / Gergely, L.; Czegledy, J.; Vaczi, L.

In: Intervirology, Vol. 13, No. 6, 1980, p. 352-356.

Research output: Contribution to journalArticle

Gergely, L. ; Czegledy, J. ; Vaczi, L. / Early nuclear antigen as DNA-binding protein in cytomegalovirus-infected cells. In: Intervirology. 1980 ; Vol. 13, No. 6. pp. 352-356.
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