Early hemorheological changes in a porcine model of intravenously given E. coli induced fulminant sepsis

N. Németh, Mariann Berhes, Ferenc Kiss, Endre Hajdu, Adam Deak, Abel Molnar, J. Szabó, B. Fülesdi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The pathophysiology of hemorheological and microcirculatory disturbances in septic process -mostly during the early hours- still not clarified in all the details, yet. In anesthetized pigs living E. coli (ATCC 25922 strain) was administered intravenously with an increasing concentration and the animals were observed for 8 hours. Before the intervention and in every 2 hours arterial (cannulated femoral artery) and venous (cannulated external jugular vein) blood samples were collected for hemorheological laboratory tests: blood and plasma viscosity, ESR, leukocyte anti-sedimentation rate, erythrocyte deformability (together with osmoscan parameters) and erythrocyte aggregation (using light-transmission and laser back-scattering methods) Control animals were stable over the 8-hour anesthesia, while septic animals died by the 6th hours in a fulminant sepsis. Over the experimental period, the tendency of impairment in erythrocyte deformability (together with osmotic gradient ektacytometry parameters) and the controversial decreasing of erythrocyte aggregation values (declining all aggregation index values, elongating t1/2) were well detected in this porcine model during the early hours (4'6) of fulminant sepsis. The in vitro effect of these bacteria on erythrocytes' micro-rheological parameter was similar: decreasing red blood cell deformability and lowering aggregation. Further studies are needed to clarify the early micro-rheological changes of bacteremia and the developing sepsis.

Original languageEnglish
Pages (from-to)479-496
Number of pages18
JournalClinical Hemorheology and Microcirculation
Volume61
Issue number3
DOIs
Publication statusPublished - Jan 4 2016

Fingerprint

Erythrocyte Aggregation
Erythrocyte Deformability
Sepsis
Swine
Escherichia coli
Erythrocytes
Blood Viscosity
Jugular Veins
Femoral Artery
Bacteremia
Lasers
Leukocytes
Anesthesia
Bacteria
Light
In Vitro Techniques

Keywords

  • Animal model
  • E. coli
  • Red blood cell aggregation
  • Red blood cell deformability
  • Sepsis

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Early hemorheological changes in a porcine model of intravenously given E. coli induced fulminant sepsis. / Németh, N.; Berhes, Mariann; Kiss, Ferenc; Hajdu, Endre; Deak, Adam; Molnar, Abel; Szabó, J.; Fülesdi, B.

In: Clinical Hemorheology and Microcirculation, Vol. 61, No. 3, 04.01.2016, p. 479-496.

Research output: Contribution to journalArticle

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