Early endocytotic steps in elicited macrophages: omega-shaped plasma membrane vesicles at their cell surface.

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Abstract

Fluid-phase and receptor-mediated endocytosis were studied in Freund's adjuvant elicited macrophages. These cells were found to bind and internalize significantly larger amounts of peroxidase-antiperoxidase (PAP) immune complex than resident macrophages. Similarly the rate of the fluid-phase uptake was higher in elicited cells. When studying the early steps of endocytotic processes, omega-shaped plasma membrane pits (d∼90 nm) were found at the macrophage cell surface. Although occurring occasionally in resident cells, their number was highly increased after elicitation in 30% of the macrophage cell population. The different morphology of these cells coincided with a lower endocytotic activity and a very strong ecto Ca2+-ATPase reaction. The present findings indicate that the elicited macrophage population is heterogenous and consists of different subclasses.

Original languageEnglish
Pages (from-to)527-538
Number of pages12
JournalCell Biology International
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 1995

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Macrophages
Cell Membrane
Calcium-Transporting ATPases
Freund's Adjuvant
Endocytosis
Antigen-Antibody Complex
Population
Peroxidase
Cell Count

ASJC Scopus subject areas

  • Cell Biology

Cite this

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abstract = "Fluid-phase and receptor-mediated endocytosis were studied in Freund's adjuvant elicited macrophages. These cells were found to bind and internalize significantly larger amounts of peroxidase-antiperoxidase (PAP) immune complex than resident macrophages. Similarly the rate of the fluid-phase uptake was higher in elicited cells. When studying the early steps of endocytotic processes, omega-shaped plasma membrane pits (d∼90 nm) were found at the macrophage cell surface. Although occurring occasionally in resident cells, their number was highly increased after elicitation in 30{\%} of the macrophage cell population. The different morphology of these cells coincided with a lower endocytotic activity and a very strong ecto Ca2+-ATPase reaction. The present findings indicate that the elicited macrophage population is heterogenous and consists of different subclasses.",
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