Early effects of cyclosporin A on in vivo oncogene expression

I. Ember, I. Kiss, E. Dezsenyi, P. Kertai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cyclosporine is a widely used immunosuppressant drug, but development of secondary malignancies has been observed after Cyclosporine containing treatments. In our study we investigated the early effect of Cyclosporin A on oncogene expression. 6, 12, 18 and 24 hours after 80 mg/bwkg Cyclosporin A treatment, RNA was isolated from different organs, slot blotted onto Hybond N+ membrane and hybridized with chemiluminescently labelled oncogene probes. As a positive control, rehybridisation with β-actin probe was used. X-ray films were exposed for 15 minutes, developed, evaluated with densitometer, and the results were expressed as a ratio to the positive control. There was no effect of Cyclosporine A on the expression of c-sis and il-2 receptor genes. The expression of N-ras, c-myc, c-ptc/ret and c-myb oncogenes was elevated in the thymus, lymph nodes, spleen and liver, either transitionally or continuously. We could not detect any elevated gene expressions in the kidney, lung and bone marrow.

Original languageEnglish
Pages (from-to)1095-1096
Number of pages2
JournalAnticancer Research
Volume14
Issue number3 A
Publication statusPublished - 1994

Fingerprint

Oncogenes
Cyclosporine
myb Genes
X-Ray Film
Immunosuppressive Agents
Thymus Gland
Actins
Spleen
Lymph Nodes
Bone Marrow
RNA
Kidney
Gene Expression
Lung
Membranes
Liver
Pharmaceutical Preparations
Genes
Neoplasms

Keywords

  • Cyclosporin A
  • Oncogene expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ember, I., Kiss, I., Dezsenyi, E., & Kertai, P. (1994). Early effects of cyclosporin A on in vivo oncogene expression. Anticancer Research, 14(3 A), 1095-1096.

Early effects of cyclosporin A on in vivo oncogene expression. / Ember, I.; Kiss, I.; Dezsenyi, E.; Kertai, P.

In: Anticancer Research, Vol. 14, No. 3 A, 1994, p. 1095-1096.

Research output: Contribution to journalArticle

Ember, I, Kiss, I, Dezsenyi, E & Kertai, P 1994, 'Early effects of cyclosporin A on in vivo oncogene expression', Anticancer Research, vol. 14, no. 3 A, pp. 1095-1096.
Ember I, Kiss I, Dezsenyi E, Kertai P. Early effects of cyclosporin A on in vivo oncogene expression. Anticancer Research. 1994;14(3 A):1095-1096.
Ember, I. ; Kiss, I. ; Dezsenyi, E. ; Kertai, P. / Early effects of cyclosporin A on in vivo oncogene expression. In: Anticancer Research. 1994 ; Vol. 14, No. 3 A. pp. 1095-1096.
@article{a492e11aee98474395f20ca8bed89cd0,
title = "Early effects of cyclosporin A on in vivo oncogene expression",
abstract = "Cyclosporine is a widely used immunosuppressant drug, but development of secondary malignancies has been observed after Cyclosporine containing treatments. In our study we investigated the early effect of Cyclosporin A on oncogene expression. 6, 12, 18 and 24 hours after 80 mg/bwkg Cyclosporin A treatment, RNA was isolated from different organs, slot blotted onto Hybond N+ membrane and hybridized with chemiluminescently labelled oncogene probes. As a positive control, rehybridisation with β-actin probe was used. X-ray films were exposed for 15 minutes, developed, evaluated with densitometer, and the results were expressed as a ratio to the positive control. There was no effect of Cyclosporine A on the expression of c-sis and il-2 receptor genes. The expression of N-ras, c-myc, c-ptc/ret and c-myb oncogenes was elevated in the thymus, lymph nodes, spleen and liver, either transitionally or continuously. We could not detect any elevated gene expressions in the kidney, lung and bone marrow.",
keywords = "Cyclosporin A, Oncogene expression",
author = "I. Ember and I. Kiss and E. Dezsenyi and P. Kertai",
year = "1994",
language = "English",
volume = "14",
pages = "1095--1096",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3 A",

}

TY - JOUR

T1 - Early effects of cyclosporin A on in vivo oncogene expression

AU - Ember, I.

AU - Kiss, I.

AU - Dezsenyi, E.

AU - Kertai, P.

PY - 1994

Y1 - 1994

N2 - Cyclosporine is a widely used immunosuppressant drug, but development of secondary malignancies has been observed after Cyclosporine containing treatments. In our study we investigated the early effect of Cyclosporin A on oncogene expression. 6, 12, 18 and 24 hours after 80 mg/bwkg Cyclosporin A treatment, RNA was isolated from different organs, slot blotted onto Hybond N+ membrane and hybridized with chemiluminescently labelled oncogene probes. As a positive control, rehybridisation with β-actin probe was used. X-ray films were exposed for 15 minutes, developed, evaluated with densitometer, and the results were expressed as a ratio to the positive control. There was no effect of Cyclosporine A on the expression of c-sis and il-2 receptor genes. The expression of N-ras, c-myc, c-ptc/ret and c-myb oncogenes was elevated in the thymus, lymph nodes, spleen and liver, either transitionally or continuously. We could not detect any elevated gene expressions in the kidney, lung and bone marrow.

AB - Cyclosporine is a widely used immunosuppressant drug, but development of secondary malignancies has been observed after Cyclosporine containing treatments. In our study we investigated the early effect of Cyclosporin A on oncogene expression. 6, 12, 18 and 24 hours after 80 mg/bwkg Cyclosporin A treatment, RNA was isolated from different organs, slot blotted onto Hybond N+ membrane and hybridized with chemiluminescently labelled oncogene probes. As a positive control, rehybridisation with β-actin probe was used. X-ray films were exposed for 15 minutes, developed, evaluated with densitometer, and the results were expressed as a ratio to the positive control. There was no effect of Cyclosporine A on the expression of c-sis and il-2 receptor genes. The expression of N-ras, c-myc, c-ptc/ret and c-myb oncogenes was elevated in the thymus, lymph nodes, spleen and liver, either transitionally or continuously. We could not detect any elevated gene expressions in the kidney, lung and bone marrow.

KW - Cyclosporin A

KW - Oncogene expression

UR - http://www.scopus.com/inward/record.url?scp=0028128568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028128568&partnerID=8YFLogxK

M3 - Article

C2 - 8074456

AN - SCOPUS:0028128568

VL - 14

SP - 1095

EP - 1096

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3 A

ER -