Early changes in rat diaphragm biology with mechanical ventilation

Gábor Z. Rácz, Ghislaine Gayan-Ramirez, Dries Testelmans, Pascal Cadot, Kristel De Paepe, E. Zádor, Frank Wuytack, Marc Decramer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

To better characterize the effects of 24-hour mechanical ventilation on diaphragm, the expression of myogenic transcription factors, myosin heavy chains, and sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps was examined in rats. In the diaphragm of mechanically ventilated animals, the mRNA of MyoD, myosin heavy chain-2a and -2b, and sarcoplasmic/endoplasmlc reticulum calcium-ATPase-1a decreased, whereas myogenin mRNA increased. In the diaphragm of anesthetized and spontaneously breathing rats, only the mRNA of MyoD and myosin heavy chain-2a decreased. MyoD and myogenin protein expression followed the changes at the mRNA, whereas the myosin heavy chain isoforms did not change. Parallel experiments involving the gastrocnemius were performed to assess the relative contribution of muscle shortening versus immobilization-induced deconditioning on muscle regulatory factor expression. Passive shortening produced no additional effects compared with immobilization-induced deconditioning. The overall changes followed a remarkably similar pattern except for MyoD protein expression, which increased in the gastrocnemius and decreased in the diaphragm while its mRNA diminished in both muscles. The early alterations in the expression of muscle protein and regulatory factors may serve as underlying molecular basis for the impaired diaphragm function seen after 24 hours of mechanical ventilation. Whether immobilization-induced deconditioning and/or passive shortening play a role in these alterations could not be fully unraveled.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume168
Issue number3
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

Diaphragm
Artificial Respiration
Myosin Heavy Chains
MyoD Protein
Messenger RNA
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Immobilization
Myogenin
Muscles
Muscle Proteins
Endoplasmic Reticulum
Protein Isoforms
Respiration
Transcription Factors

Keywords

  • Diaphragm
  • Mechanical ventilation
  • Myogenic regulatory factors
  • Myosin heavy chain
  • Passive shortening

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Rácz, G. Z., Gayan-Ramirez, G., Testelmans, D., Cadot, P., De Paepe, K., Zádor, E., ... Decramer, M. (2003). Early changes in rat diaphragm biology with mechanical ventilation. American Journal of Respiratory and Critical Care Medicine, 168(3), 297-304. https://doi.org/10.1164/rccm.200206-541OC

Early changes in rat diaphragm biology with mechanical ventilation. / Rácz, Gábor Z.; Gayan-Ramirez, Ghislaine; Testelmans, Dries; Cadot, Pascal; De Paepe, Kristel; Zádor, E.; Wuytack, Frank; Decramer, Marc.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 168, No. 3, 01.08.2003, p. 297-304.

Research output: Contribution to journalArticle

Rácz, GZ, Gayan-Ramirez, G, Testelmans, D, Cadot, P, De Paepe, K, Zádor, E, Wuytack, F & Decramer, M 2003, 'Early changes in rat diaphragm biology with mechanical ventilation', American Journal of Respiratory and Critical Care Medicine, vol. 168, no. 3, pp. 297-304. https://doi.org/10.1164/rccm.200206-541OC
Rácz, Gábor Z. ; Gayan-Ramirez, Ghislaine ; Testelmans, Dries ; Cadot, Pascal ; De Paepe, Kristel ; Zádor, E. ; Wuytack, Frank ; Decramer, Marc. / Early changes in rat diaphragm biology with mechanical ventilation. In: American Journal of Respiratory and Critical Care Medicine. 2003 ; Vol. 168, No. 3. pp. 297-304.
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