Dystroglycan is involved in laminin-1-stimulated motility of Müller glial cells: Combined velocity and directionality analysis

Elöd Méhes, András Czirók, Balázs Hegedüs, Bálint Szabó, Tamás Vicsek, Jakob Satz, Kevin Campbell, Veronika Jancsik

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We investigate the role of dystroglycan, a major laminin-1 receptor and central member of the dystrophin-glycoprotein complex, in the laminin-1 induced motility of cultured Müller glial cells. Binding of laminin-1 to dystroglycan was prevented by IIH6, a function-blocking monoclonal antibody against α-dystroglycan. As an alternative means of inhibition, we used heparin to mask the dystroglycan binding site of the laminin-1, known to overlap with heparin binding sites. Cell motility was characterized in a two-dimensional motility assay based on computer-controlled videomicroscopy and statistical analysis of cellular trajectories. We obtained data on both the cell velocity and the diffusion index, a measure of direction-changing frequency. Both means of inhibition of dystroglycan function led to a significant decrease in the ability of laminin-1 to stimulate cell migration. At the same time, dystroglycan function does not appear to be involved in laminin-1-dependent increase in process dynamism and direction-changing activity.

Original languageEnglish
Pages (from-to)492-500
Number of pages9
JournalGlia
Volume49
Issue number4
DOIs
Publication statusPublished - Mar 2005

Keywords

  • Dystrophin glycoprotein complex
  • Extracellular matrix
  • Persistence
  • Statistical analysis
  • Videomicroscopy

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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