Dynamism of the development of "Fc receptor-blocking antibodies" after immunization with buffy coat.

I. B. Petri, E. Gyódi, G. Kaiser, M. Kassai, G. G. Petrányi

Research output: Contribution to journalArticle

Abstract

Eight healthy male blood donors who had not received transfusion previously, were immunized 5 times at 6-week intervals. The immunization was carried out with "buffy coat" separated from 400 ml freshly obtained donor blood. Donor-recipient pairs showed a mismatching for 1 to 4 HLA antigens. The production of HLA antibodies could be demonstrated in 4 patients during the repeated immunizations. Fc receptor-blocking antibody activity was determined with the erythrocyte antibody inhibition (EAI) method in serum samples for the recipients. An increase in the Fc receptor-blocking antibody serum level could be detected already on the second day after the first immune stimulus. Fc receptor-blocking activity was maximum on the 10th day after immunization. After repeated immune stimuli, the increased non-specific Fc receptor-blocking antibody activity was demonstrated independently of the appearance of specific HLA cytotoxic antibodies.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalHaematologia
Volume22
Issue number3
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Hematology

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