Beyond static properties, like primary sequence, folding, electrostatics, etc. protein action is also influenced by dynamic factors, which may essentially influence structure and function. In this chapter first we give a short outline of various methods for the computer modelling of protein dynamics, then discuss the most important experimental technique, nuclear magnetic resonance spectroscopy applied to proteins. Combined application of these two methods will be illustrated on two small proteins, extensively studied in our laboratory. First we discuss the Trp-cage protein, to our knowledge the smallest one with essential attributes of basic protein properties. Our second case study deals with podocin, an interesting small protein. Its dynamic properties determine potential malfunction, the inheritance of a specific kidney disease.
|Title of host publication||Protein Modelling|
|Publisher||Springer International Publishing|
|Number of pages||26|
|ISBN (Print)||3319099752, 9783319099750|
|Publication status||Published - Jul 1 2014|
ASJC Scopus subject areas