Dynamics and localization of H2O2 production in elicited plant cells

K. Bóka, N. Orbán, Z. Kristóf

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

H2O2 produced in plant cells plays a dual role. In addition to its antimicrobial effect, it also acts as a secondary messenger initiating and modulating responses of plants exposed to unfavorable external signals. A suspension culture of Rubia tinctorum cells challenged with elicitors was used as a model system to investigate H2O2 formation. Cellular H2O2 was measured by a modified titanium(IV) method, while that in the medium was detected with scopoletin fluorescence. Localization of H2O2 production at the ultrastructural level was carried out by the CeCl3 reaction. A fungal elicitor induced H2O2 production with transient maxima, the first of which appeared 4 min after treatment. Three subsequent maxima appeared in the cells up to 48 h after treatment. Exposure of cells to exogenous jasmonic acid and salicylic acid also changed the H2O2 concentration maxima over 48 h; however, their timing was slightly shifted. Fungal-elicitor, jasmonic acid, and salicylic acid treatments had different effects on the H 2O2 concentration in the medium. Ultrastructural investigations revealed that electron-dense precipitates were present at the plasmalemma and in some nearby vesicular cytoplasmic structures 30 min after treatment. Later samples showed cytochemical-precipitate accumulation in the cell walls. These deposits appeared to be local and independent of the direction of the external signal. We could not detect the presence of H2O 2 in peroxisomes, mitochondria, plastids, or the central vacuolar space. Electron energy loss spectroscopy investigations distinguished between the cerium-containing precipitates and other electrondense particles, thereby proving that H2O2 generation occurs locally.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalProtoplasma
Volume230
Issue number1-2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Plant Cells
Salicylic Acid
Rubia
Electron Energy-Loss Spectroscopy
jasmonic acid
Scopoletin
salicylic acid
Cytoplasmic Structures
Cerium
Plastids
Rubia tinctorum
Peroxisomes
electrons
cells
cerium
Titanium
titanium dioxide
scopoletin
Cell Wall
Suspensions

Keywords

  • Electron energy loss spectroscopy
  • Elicitor
  • Hydrogen peroxide
  • Rubia tinctorum
  • Spectrofluorometry
  • Spectrophotometry

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Dynamics and localization of H2O2 production in elicited plant cells. / Bóka, K.; Orbán, N.; Kristóf, Z.

In: Protoplasma, Vol. 230, No. 1-2, 03.2007, p. 89-97.

Research output: Contribution to journalArticle

Bóka, K. ; Orbán, N. ; Kristóf, Z. / Dynamics and localization of H2O2 production in elicited plant cells. In: Protoplasma. 2007 ; Vol. 230, No. 1-2. pp. 89-97.
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