Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells

G. Kozma, Fabrizio Martelli, Maria Verrucci, Laura Gutiérrez, Giovanni Migliaccio, Massimo Sanchez, Elena Alfani, Sjaak Philipsen, Anna Rita Migliaccio

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Abstract

Objectives: To identify the regulatory sequences driving Gata1 expression in conventional dendritic cells (cDC). Materials and Methods: The number and expression levels of Gata1, Gata1-target genes and hypersensitive site (HS) 2 (the eosinophil-specific enhancer)-driven green fluorescent protein (GFP) reporter of cDCs from mice lacking HS1 (the erythroid/megakaryocytic-specific enhancer, Gata1low mutation) and wild-type littermates, as well as the response to lipopolysaccharide of ex vivo-generated wild-type and Gata1low DCs were investigated. Results: cDC maturation was associated with bell-shaped changes in Gata1 expression that peaked in cDCs precursors from blood. The Gata1low mutation did not affect Gata1 expression in cDC precursors and these cells expressed the HS2-driven reporter, indicating that Gata1 expression is HS2-driven in these cells. By contrast, the Gata1low mutation reduced Gata1 expression in mature cDCs and these cells did not express GFP, indicating that mature cDCs express Gata1 driven by HS1. In blood, the number of cDC precursors expressing CD40/CD80 was reduced in Gata1low mice, while CD40pos/CD80pos cDC precursors from wild-type mice expressed the HS2-GFP reporter, suggesting that Gata1 expression in these cells is both HS1- and HS2-driven. In addition, the antigen and accessory molecules presentation process induced by lipopolysaccharide in ex vivo-generated wild-type DC was associated with increased acetylated histone 4 occupancy of HS1, while ex vivo-generated Gata1low cDCs failed to respond to lipopolysaccharide, suggesting that HS1 activation is required for cDC maturation. Conclusion: These results identify a dynamic pattern of Gata1 regulation that switches from an HS1 to an HS2-dependent phase during the maturation of cDCs associated with the antigen-presentation process in the blood.

Original languageEnglish
JournalExperimental Hematology
Volume38
Issue number6
DOIs
Publication statusPublished - Jun 2010

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Dendritic Cells
Green Fluorescent Proteins
Lipopolysaccharides
Mutation
Antigen Presentation
Eosinophils
Histones
Antigens
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology

Cite this

Kozma, G., Martelli, F., Verrucci, M., Gutiérrez, L., Migliaccio, G., Sanchez, M., ... Migliaccio, A. R. (2010). Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells. Experimental Hematology, 38(6). https://doi.org/10.1016/j.exphem.2010.03.006

Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells. / Kozma, G.; Martelli, Fabrizio; Verrucci, Maria; Gutiérrez, Laura; Migliaccio, Giovanni; Sanchez, Massimo; Alfani, Elena; Philipsen, Sjaak; Migliaccio, Anna Rita.

In: Experimental Hematology, Vol. 38, No. 6, 06.2010.

Research output: Contribution to journalArticle

Kozma, G, Martelli, F, Verrucci, M, Gutiérrez, L, Migliaccio, G, Sanchez, M, Alfani, E, Philipsen, S & Migliaccio, AR 2010, 'Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells', Experimental Hematology, vol. 38, no. 6. https://doi.org/10.1016/j.exphem.2010.03.006
Kozma, G. ; Martelli, Fabrizio ; Verrucci, Maria ; Gutiérrez, Laura ; Migliaccio, Giovanni ; Sanchez, Massimo ; Alfani, Elena ; Philipsen, Sjaak ; Migliaccio, Anna Rita. / Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells. In: Experimental Hematology. 2010 ; Vol. 38, No. 6.
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abstract = "Objectives: To identify the regulatory sequences driving Gata1 expression in conventional dendritic cells (cDC). Materials and Methods: The number and expression levels of Gata1, Gata1-target genes and hypersensitive site (HS) 2 (the eosinophil-specific enhancer)-driven green fluorescent protein (GFP) reporter of cDCs from mice lacking HS1 (the erythroid/megakaryocytic-specific enhancer, Gata1low mutation) and wild-type littermates, as well as the response to lipopolysaccharide of ex vivo-generated wild-type and Gata1low DCs were investigated. Results: cDC maturation was associated with bell-shaped changes in Gata1 expression that peaked in cDCs precursors from blood. The Gata1low mutation did not affect Gata1 expression in cDC precursors and these cells expressed the HS2-driven reporter, indicating that Gata1 expression is HS2-driven in these cells. By contrast, the Gata1low mutation reduced Gata1 expression in mature cDCs and these cells did not express GFP, indicating that mature cDCs express Gata1 driven by HS1. In blood, the number of cDC precursors expressing CD40/CD80 was reduced in Gata1low mice, while CD40pos/CD80pos cDC precursors from wild-type mice expressed the HS2-GFP reporter, suggesting that Gata1 expression in these cells is both HS1- and HS2-driven. In addition, the antigen and accessory molecules presentation process induced by lipopolysaccharide in ex vivo-generated wild-type DC was associated with increased acetylated histone 4 occupancy of HS1, while ex vivo-generated Gata1low cDCs failed to respond to lipopolysaccharide, suggesting that HS1 activation is required for cDC maturation. Conclusion: These results identify a dynamic pattern of Gata1 regulation that switches from an HS1 to an HS2-dependent phase during the maturation of cDCs associated with the antigen-presentation process in the blood.",
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AU - Migliaccio, Giovanni

AU - Sanchez, Massimo

AU - Alfani, Elena

AU - Philipsen, Sjaak

AU - Migliaccio, Anna Rita

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