Dynamic imaging and spatially localized ESR spectroscopy of oriented phospholipid multilayers

T. Páli, B. Ebert, L. I. Horváth

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Abstract

Dynamic ESR imaging of various patterns (i.e., concentration profiles) of spin-labeled lipids in dimyristoylphosphatidylcholine multilayers provides a novel technique for macroscopic lateral-diffusion measurements at a spatial resolution of ∼50 μm. It is shown in this study that the use of the modulated-field-gradient method allows the simultaneous detection of one-dimensional spatial images and spatially localized ESR spectra of different regions of the sample. The spectral effects of local dehydration at the rim of multilayered samples is discussed, separating it from the fully hydrated region in the center and assigning the broad spectrum component to strong spin dipole-dipole interaction in this region. A new method is recommended for the analysis of dynamically rearranging images and a lipid lateral-diffusion coefficient of ∼6 × 10-8 cm2 s-1 is obtained from the filling of narrow concentration gaps.

Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalJournal of Magnetic Resonance (1969)
Volume96
Issue number3
DOIs
Publication statusPublished - Feb 15 1992

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