Dynamic aspects of the incorporation of proteins into biological membranes

L. I. Horváth, M. Török, K. Hideg, L. Dux

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The contributions of intramembranous and extramembranous segments of transmembrane proteins to frictional forces have been studied by covalently attached 14N- and 15N-indane dione and maleimide spin labels using saturation transfer electron spin resonance spectroscopy. The role of molecular size and membrane viscosity is discussed in determining rotational mobilities of proteins. By comparing the measured rotational correlation times with the predictions of hydrodynamic models the aggregation states of transmembrane proteins is estimated. On increasing the viscosity of the aqueous phase by polyols the viscous drag of the extramembranous segments of proteins is increased and from systematic hydrodynamic measurements the size of the protruding segments can be estimated. The role of slowed molecular diffusion is briefly discussed in the inhibition of enzymatic activity.

Original languageEnglish
Pages (from-to)188-193
Number of pages6
JournalJournal of Molecular Recognition
Volume10
Issue number4
DOIs
Publication statusPublished - Jul 1997

Fingerprint

Biological membranes
Proteins
Membranes
Hydrodynamics
Viscosity
Electron spin resonance spectroscopy
Spin Labels
Polyols
Electron Spin Resonance Spectroscopy
Drag
Labels
Agglomeration

Keywords

  • Rotational mobility
  • Saturation transfer electron spin resonance spectroscopy
  • Viscous modulation

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Computer Vision and Pattern Recognition
  • Immunology
  • Molecular Biology

Cite this

Dynamic aspects of the incorporation of proteins into biological membranes. / Horváth, L. I.; Török, M.; Hideg, K.; Dux, L.

In: Journal of Molecular Recognition, Vol. 10, No. 4, 07.1997, p. 188-193.

Research output: Contribution to journalArticle

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