Dural administration of inflammatory soup or Complete Freund’s Adjuvant induces activation and inflammatory response in the rat trigeminal ganglion

M. Lukács, K. A. Haanes, Zs Majláth, J. Tajti, L. Vécsei, K. Warfvinge, L. Edvinsson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Migraine is a painful disorder with a huge impact on individual and public health. We hypothesize that migraine pain originates from a central mechanism that results secondarily in hypersensitivity in peripheral afferents associated with the cerebral and cranial blood vessels. It has previously been shown that application of inflammatory or algesic substances onto the dura mater or chemical stimulation of the dural receptive fields causes hypersensitivity to mechanical and thermal stimulation together with direct activation of the TG. We asked whether local inflammation of dura mater induces inflammatory activation in the trigeminal ganglion. Methods: We performed topical administration of inflammatory soup (IS) or Complete Freund’s Adjuvant (CFA) onto an exposed area of the rat dura mater in vivo for 20 min. The window was closed and the rats were sacrificed after 4 h and up to 7 days. Myography was performed on middle meningeal arteries. The trigeminal ganglia were removed and processed for immunohistochemistry or Western blot. Results: Both CFA and IS induced enhanced expression of pERK1/2, IL-1β and CGRP in the trigeminal ganglia. The pERK1/2 immunoreactivity was mainly seen in the satellite glial cells, while IL-1β reactivity was observed in the neuronal cytoplasm, close to the cell membrane, seemingly as sign of neuro-glial interaction. The CGRP expression in the neurons and nerve fibres was enhanced after the application of either inflammatory agent. Myography resulted in a strong vasoconstrictor response to IS, but not to CFA. Conclusions: These results suggest that the application of IS or CFA onto the dura mater causes long-term activation of the TG and demonstrate the importance of the neuro-glial interaction in the activation of the trigeminovascular system.

Original languageEnglish
Article number79
JournalThe journal of headache and pain
Volume16
Issue number1
DOIs
Publication statusPublished - Dec 7 2015

Fingerprint

Dura Mater
Trigeminal Ganglion
Freund's Adjuvant
Myography
Neuroglia
Migraine Disorders
Interleukin-1
Hypersensitivity
Meningeal Arteries
Chemical Stimulation
Topical Administration
Vasoconstrictor Agents
Nerve Fibers
Blood Vessels
Cytoplasm
Public Health
Hot Temperature
Western Blotting
Immunohistochemistry
Cell Membrane

Keywords

  • CGRP
  • Complete Freund’s Adjuvant
  • Dura mater
  • IL-1β
  • Inflammatory soup
  • pERK1/2
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Clinical Neurology

Cite this

Dural administration of inflammatory soup or Complete Freund’s Adjuvant induces activation and inflammatory response in the rat trigeminal ganglion. / Lukács, M.; Haanes, K. A.; Majláth, Zs; Tajti, J.; Vécsei, L.; Warfvinge, K.; Edvinsson, L.

In: The journal of headache and pain, Vol. 16, No. 1, 79, 07.12.2015.

Research output: Contribution to journalArticle

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abstract = "Background: Migraine is a painful disorder with a huge impact on individual and public health. We hypothesize that migraine pain originates from a central mechanism that results secondarily in hypersensitivity in peripheral afferents associated with the cerebral and cranial blood vessels. It has previously been shown that application of inflammatory or algesic substances onto the dura mater or chemical stimulation of the dural receptive fields causes hypersensitivity to mechanical and thermal stimulation together with direct activation of the TG. We asked whether local inflammation of dura mater induces inflammatory activation in the trigeminal ganglion. Methods: We performed topical administration of inflammatory soup (IS) or Complete Freund’s Adjuvant (CFA) onto an exposed area of the rat dura mater in vivo for 20 min. The window was closed and the rats were sacrificed after 4 h and up to 7 days. Myography was performed on middle meningeal arteries. The trigeminal ganglia were removed and processed for immunohistochemistry or Western blot. Results: Both CFA and IS induced enhanced expression of pERK1/2, IL-1β and CGRP in the trigeminal ganglia. The pERK1/2 immunoreactivity was mainly seen in the satellite glial cells, while IL-1β reactivity was observed in the neuronal cytoplasm, close to the cell membrane, seemingly as sign of neuro-glial interaction. The CGRP expression in the neurons and nerve fibres was enhanced after the application of either inflammatory agent. Myography resulted in a strong vasoconstrictor response to IS, but not to CFA. Conclusions: These results suggest that the application of IS or CFA onto the dura mater causes long-term activation of the TG and demonstrate the importance of the neuro-glial interaction in the activation of the trigeminovascular system.",
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AU - Haanes, K. A.

AU - Majláth, Zs

AU - Tajti, J.

AU - Vécsei, L.

AU - Warfvinge, K.

AU - Edvinsson, L.

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