Dual (excitatory and inhibitory) calretinin innervation of AMPA receptor-containing neurons in the rat lateral septum

N. Holderith, Frederique Varoqueaux, Z. Borhegyi, Csaba Leranth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A recent study demonstrated both an extrinsic and an intrinsic calretinin (CR) innervation of the rat septal complex and that a population of the extrinsic calretinin fibers is aspartate/glutamate-containing. The aim of this study was to determine which types (GluR1, GluR2/3, or both) of AMPA receptor-containing lateral septal area neurons are innervated by extrinsic and intrinsic CR neurons and whether the intrinsic CR cells are GABAergic. Light- and electron-microscopic single immunostaining for CR, GluR1, and GluR2/3, as well as light- and electron-microscopic-double immunostaining experiments for CR plus GluR1 and CR plus GluR2/3 were performed in the lateral septal area. Furthermore, the 'mirror' colocalization technique was employed on consecutive vibratome sections of the septal complex to investigate whether the intrinsic septal CR neurons are GABAergic. The results are summarized as follows: (1) both GluR1- and GluR2/3-immunoreactive neurons are innervated by CR-containing fibers; (2) the majority of these synapses, observed mainly on the soma and, to a lesser extent, on proximal dendrites of AMPA receptor-containing neurons, represent asymmetric synaptic membrane specializations; (3) a minority of CR-containing axon terminals associated with both GluR1- and GluR2/3-immunoreactive neurons form symmetric contacts, predominantly on their soma; and (4) 93% of the lateral septal area CR cells are GABAergic. These observations indicate that both GluR1- and GluR2/3-containing lateral septal area neurons receive a dual intrinsic and extrinsic CR innervation. The former (intrinsic) CR boutons are GABAergic, while the latter form asymmetric synaptic contacts, are excitatory, and probably originate in the supramammillary area, since previous work has demonstrated that a population of supramammillo-septal fibers contain aspartate and/or glutamate.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalExperimental Brain Research
Volume119
Issue number1
DOIs
Publication statusPublished - 1998

Fingerprint

Calbindin 2
AMPA Receptors
Neurons
Septum of Brain
Carisoprodol
Aspartic Acid
Glutamic Acid
Electrons
Light
GABAergic Neurons
Synaptic Membranes
Presynaptic Terminals
Dendrites
Synapses
Population

Keywords

  • Colocalization
  • Double immunostaining
  • GABA
  • Glutamate receptor
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dual (excitatory and inhibitory) calretinin innervation of AMPA receptor-containing neurons in the rat lateral septum. / Holderith, N.; Varoqueaux, Frederique; Borhegyi, Z.; Leranth, Csaba.

In: Experimental Brain Research, Vol. 119, No. 1, 1998, p. 65-72.

Research output: Contribution to journalArticle

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