D20 discriminates gamma aminobutyric acid A receptors with different lifetimes

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Abstract

Progress of gamma aminobutyric acidA receptor channel (GABAAR) mediated 36Cf~ ion uptake by native membrane vesicles from the rat cerebral cortex has been examined using rapid kinetic techniques. Data on GABA responsive 36C1" ion flux, measured in a series of buffered salt solutions made up of different H20-D20 mixtures, were evaluated by fitting a model with the mechanisms of channel opening and desensitization of GABAAR. The overall isotope effect could be isolated into its component contributions from the GABAARs by their different active lifetimes. Ion flux mediated by the slower desensitizing GABAAR was completely inhibited by 20% D20. Large and selective D20 effects on ion fluxes mediated by the kinetically distinguishable native GABAARs suggest distinct types of subunit combinations for these receptors.

Original languageEnglish
Pages (from-to)1124-1126
Number of pages3
JournalNeuroreport
Volume3
Issue number12
DOIs
Publication statusPublished - Dec 1992

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Keywords

  • 36C1- flux
  • D20
  • GABA
  • Native membrane vesicles
  • Rat cerebral cortex

ASJC Scopus subject areas

  • Neuroscience(all)

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