DSC study of glycerol-extracted muscle fibers in intermediate states of ATP hydrolysis

T. Dergez, F. Konczol, N. Farkas, J. Belágyi, D. Lőrinczy

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The heat capacity of contractile proteins actin and myosin was studied in psoas muscle of rabbit in strongly and weakly binding state of myosin to actin as a function of temperature by DSC. Deconvolution of the unfolding scans makes possible to characterize the structural domains of the macromolecules. We tried to approach the unfolding process in different intermediate state of ATP hydrolysis. The thermal transitions were calorimetrically irreversible, therefore the two-state irreversible model that describes fairly well the denaturation of different proteins was used for evaluation of the denaturation processes in muscle fibers in strongly (rigor, ADP) and weakly binding states (ATP·Vi, ADP·AlF4) of myosin to actin. Deconvolution resulted in four transitions, the first three transition temperatures were almost independent of the intermediate states of muscle, the last transition temperature was shifted to higher temperature, when the buffer solution was manipulated. The mean values in strongly binding states were T m1=52.9±0.7°C, Tm2=57.9±0. 7°C, Tm3=63.7±1.0°C and T m4=67.8±0.7°C, but the last transition increased to higher temperature depending on the Pi analogue.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalJournal of Thermal Analysis and Calorimetry
Volume80
Issue number2
DOIs
Publication statusPublished - May 2005

Fingerprint

muscle fibers
myosins
adenosine triphosphate
Adenosinetriphosphate
Myosins
glycerols
Glycerol
Muscle
hydrolysis
Actins
Hydrolysis
Denaturation
Adenosine Triphosphate
biopolymer denaturation
Deconvolution
muscles
Superconducting transition temperature
Fibers
transition temperature
proteins

Keywords

  • ATP hydrolysis
  • Deconvolution of DSC scans
  • DSC
  • Muscle fiber

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

DSC study of glycerol-extracted muscle fibers in intermediate states of ATP hydrolysis. / Dergez, T.; Konczol, F.; Farkas, N.; Belágyi, J.; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 80, No. 2, 05.2005, p. 445-449.

Research output: Contribution to journalArticle

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