DSC measurement of cartilage destruction caused by septic arthritis

T. Sillinger, P. Than, B. Kocsis, D. Lőrinczy

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Treatment of a bacterial arthritis is a challenging task for a clinician as inadequate therapy can cause cartilage destruction and can result in severe osteoarthritis of the affected joint. The development of cartilage destruction in septic arthritis is not known in details. The aim of this study was to follow this process by calorimetric method. We induced experimental septic arthritis in knee joints of seven New Zealand rabbits by single inocculation of Staphylococcus aureusOKI 112001 culture (1.5 mL 8•108±5% c.f.u.). The first rabbit died on the 11thday. At that time all the other subjects were made overslept and samples were isolated from the cartilage of the femurs for calorimetric measurement. The DSC scans clearly demonstrated the development of infective structural destruction in cartilage from the first to the tenth day of incubation. In case of healthy control the melting temperatures (T m) were: 49.7, 55 and 63.4°C and the total calorimetric enthalpy change (ΔH) was 0.55 J g-1. After the first day the enthalpy decreased (0.375 J g-1), the first two transition temperature shifted towards higher temperature: 57 and 63.15°C. Up to the fourth day the effect of infection culminated with T mof 49.3, 55.9, 59.4, 62.8°C and further decrease of the Delta;H. At the fifth day the effect of infection is culminated in two separable thermal denaturation events (with 55 and 63.3°C T ms) with high jump in ΔHindicating the dramatic change of the structure of rabbit cartilage, so this time elapsed seems to be critical from the point of view of practical clinical relevance too. Between the 7thand 11thdays practically we had same melting temperatures (50 and 63°C) with low (~0.24 J g-1) enthalpy.

Original languageEnglish
Pages (from-to)221-223
Number of pages3
JournalJournal of Thermal Analysis and Calorimetry
Volume82
Issue number1
DOIs
Publication statusPublished - Sep 2005

Fingerprint

arthritis
cartilage
Cartilage
destruction
rabbits
Enthalpy
enthalpy
infectious diseases
Melting point
melting
femur
Denaturation
staphylococcus
biopolymer denaturation
New Zealand
Superconducting transition temperature
therapy
transition temperature
temperature
causes

Keywords

  • DSC
  • Rabbit cartilage
  • Septic arthritis
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

DSC measurement of cartilage destruction caused by septic arthritis. / Sillinger, T.; Than, P.; Kocsis, B.; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 82, No. 1, 09.2005, p. 221-223.

Research output: Contribution to journalArticle

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