Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines

M. Rakszegi, E. Darkó, Alison Lovegrove, István Molnár, L. Láng, Z. Bedő, M. Molnár-Láng, Peter Shewry

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Wild relatives of wheat, such as Aegilops spp. are potential sources of genes conferring tolerance to drought stress. As drought stress affects seed composition, the main goal of the present study was to determine the effects of drought stress on the content and composition of the grain storage protein (gliadin (Gli), glutenin (Glu), unextractable polymeric proteins (UPP%) and dietary fiber (arabinoxylan, β-glucan) components of hexaploid bread wheat (T. aestivum) lines containing added chromosomes from Ae. biuncialisor Ae. geniculata. Both Aegilops parents have higher contents of protein and β-glucan and higher proportions of water-soluble arabinoxylans (determined as pentosans) than wheat when grown under both well-watered and drought stress conditions. In general, drought stress resulted in increased contents of protein and total pentosans in the addition lines, while the β-glucan content decreased in many of the addition lines. The differences found between the wheat/Aegilops addition lines and wheat parents under well-watered conditions were also manifested under drought stress conditions: Namely, elevated β-glucan content was found in addition lines containing chromosomes 5U g , 7U g and 7M b , while chromosomes 1U b and 1M g affected the proportion of polymeric proteins (determined as Glu/Gli and UPP%, respectively) under both well-watered and drought stress conditions. Furthermore, the addition of chromosome 6M g decreased the WE-pentosan content under both conditions. The grain composition of the Aegilops accessions was more stable under drought stress than that of wheat, and wheat lines with the added Aegilops chromosomes 2M g and 5M g also had more stable grain protein and pentosan contents. The negative effects of drought stress on both the physical and compositional properties of wheat were also reduced by the addition of these. These results suggest that the stability of the grain composition could be improved under drought stress conditions by the intraspecific hybridization of wheat with its wild relatives.

Original languageEnglish
Article numbere0211892
JournalPLoS ONE
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 1 2019

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whole grain flour
Aegilops
Drought
Droughts
Dietary Fiber
Flour
Heat-Shock Proteins
fiber content
wheat flour
Triticum
dietary fiber
water stress
wheat
pentosans
Chromosomes
Glucans
glucans
proteins
Proteins
chromosomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines. / Rakszegi, M.; Darkó, E.; Lovegrove, Alison; Molnár, István; Láng, L.; Bedő, Z.; Molnár-Láng, M.; Shewry, Peter.

In: PLoS ONE, Vol. 14, No. 2, e0211892, 01.02.2019.

Research output: Contribution to journalArticle

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