Drosophila Nimrod proteins bind bacteria

János Zsámboki, Gábor Csordás, Viktor Honti, Lajos Pintér, Izabella Bajusz, László Galgóczy, I. Andó, E. Kurucz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Engulfment of foreign particles by phagocytes is initiated by the engagement of phagocytic receptors. We have previously reported that NimC1 is involved in the phagocytosis of bacteria in Drosophila melanogaster. We have identified a family of genes, the Nimrod gene superfamily, encoding characteristic NIM domain containing structural homologues of NimC1. In this work we studied the bacterium-binding properties of the Nimrod proteins by using a novel immunofluorescencebased flow cytometric assay. This method proved to be highly reproducible and suitable for investigations of the bacteriumbinding capacities of putative phagocytosis receptors. We found that NimC1, NimA, NimB1 and NimB2 bind bacteria significantly but differently. In this respect they are similar to other NIM domain containing receptors Eater and Draper.

Original languageEnglish
Pages (from-to)633-645
Number of pages13
JournalCentral European Journal of Biology
Volume8
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

Drosophila Proteins
Drosophila
Bacteria
phagocytosis
receptors
bacteria
Genes
Proteins
proteins
binding properties
phagocytes
Phagocytes
Drosophila melanogaster
Phagocytosis
Assays
genes
assays
bupirimate
methodology

Keywords

  • Drosophila
  • Innate immunity
  • Phagocytosis
  • Receptor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Zsámboki, J., Csordás, G., Honti, V., Pintér, L., Bajusz, I., Galgóczy, L., ... Kurucz, E. (2013). Drosophila Nimrod proteins bind bacteria. Central European Journal of Biology, 8(7), 633-645. https://doi.org/10.2478/s11535-013-0183-4

Drosophila Nimrod proteins bind bacteria. / Zsámboki, János; Csordás, Gábor; Honti, Viktor; Pintér, Lajos; Bajusz, Izabella; Galgóczy, László; Andó, I.; Kurucz, E.

In: Central European Journal of Biology, Vol. 8, No. 7, 2013, p. 633-645.

Research output: Contribution to journalArticle

Zsámboki, J, Csordás, G, Honti, V, Pintér, L, Bajusz, I, Galgóczy, L, Andó, I & Kurucz, E 2013, 'Drosophila Nimrod proteins bind bacteria', Central European Journal of Biology, vol. 8, no. 7, pp. 633-645. https://doi.org/10.2478/s11535-013-0183-4
Zsámboki J, Csordás G, Honti V, Pintér L, Bajusz I, Galgóczy L et al. Drosophila Nimrod proteins bind bacteria. Central European Journal of Biology. 2013;8(7):633-645. https://doi.org/10.2478/s11535-013-0183-4
Zsámboki, János ; Csordás, Gábor ; Honti, Viktor ; Pintér, Lajos ; Bajusz, Izabella ; Galgóczy, László ; Andó, I. ; Kurucz, E. / Drosophila Nimrod proteins bind bacteria. In: Central European Journal of Biology. 2013 ; Vol. 8, No. 7. pp. 633-645.
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