Drosophila Arl8 is a general positive regulator of lysosomal fusion events

Attila Boda, Péter Lőrincz, Szabolcs Takáts, Tamás Csizmadia, Sarolta Tóth, A. Kovács, G. Juhász

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.

Original languageEnglish
Pages (from-to)533-544
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1866
Issue number4
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Lysosomes
Drosophila
Monomeric GTP-Binding Proteins
Endosomes
Autophagy
Secretory Vesicles
Salivary Glands
Adipocytes

Keywords

  • Arl8
  • Autophagy
  • Crinophagy
  • Endosome
  • Fusion
  • Lysosome

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Drosophila Arl8 is a general positive regulator of lysosomal fusion events. / Boda, Attila; Lőrincz, Péter; Takáts, Szabolcs; Csizmadia, Tamás; Tóth, Sarolta; Kovács, A.; Juhász, G.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1866, No. 4, 01.04.2019, p. 533-544.

Research output: Contribution to journalArticle

Boda, Attila ; Lőrincz, Péter ; Takáts, Szabolcs ; Csizmadia, Tamás ; Tóth, Sarolta ; Kovács, A. ; Juhász, G. / Drosophila Arl8 is a general positive regulator of lysosomal fusion events. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2019 ; Vol. 1866, No. 4. pp. 533-544.
@article{a7b82d3b679b4e6596d2b813a047d89a,
title = "Drosophila Arl8 is a general positive regulator of lysosomal fusion events",
abstract = "The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.",
keywords = "Arl8, Autophagy, Crinophagy, Endosome, Fusion, Lysosome",
author = "Attila Boda and P{\'e}ter Lőrincz and Szabolcs Tak{\'a}ts and Tam{\'a}s Csizmadia and Sarolta T{\'o}th and A. Kov{\'a}cs and G. Juh{\'a}sz",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.bbamcr.2018.12.011",
language = "English",
volume = "1866",
pages = "533--544",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Drosophila Arl8 is a general positive regulator of lysosomal fusion events

AU - Boda, Attila

AU - Lőrincz, Péter

AU - Takáts, Szabolcs

AU - Csizmadia, Tamás

AU - Tóth, Sarolta

AU - Kovács, A.

AU - Juhász, G.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.

AB - The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.

KW - Arl8

KW - Autophagy

KW - Crinophagy

KW - Endosome

KW - Fusion

KW - Lysosome

UR - http://www.scopus.com/inward/record.url?scp=85059445015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059445015&partnerID=8YFLogxK

U2 - 10.1016/j.bbamcr.2018.12.011

DO - 10.1016/j.bbamcr.2018.12.011

M3 - Article

C2 - 30590083

AN - SCOPUS:85059445015

VL - 1866

SP - 533

EP - 544

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 4

ER -