Drosophila Arl8 is a general positive regulator of lysosomal fusion events

Attila Boda, Péter Lőrincz, Szabolcs Takáts, Tamás Csizmadia, Sarolta Tóth, A. Kovács, G. Juhász

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5 Citations (Scopus)

Abstract

The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.

Original languageEnglish
Pages (from-to)533-544
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1866
Issue number4
DOIs
Publication statusPublished - Apr 1 2019

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Keywords

  • Arl8
  • Autophagy
  • Crinophagy
  • Endosome
  • Fusion
  • Lysosome

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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