Down-regulation of RpS21, a putative translation initiation factor interacting with P40, produces viable minute imagos and lineal lethality with overgrown hematopoietic organs and imaginal discs

István Török, Daniela Herrmann-Horle, István Kiss, Gabriela Tick, Gábor Speer, Rolf Schmitt, Bernard M. Mechler

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.

Original languageEnglish
Pages (from-to)2308-2321
Number of pages14
JournalMolecular and cellular biology
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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