Down-regulation of P-glycoprotein is associated with resistance to cisplatin and VP-16 in human lung cancer cell lines

Jiarui Wang, Huiling Wang, Long Zhao, Sufang Fan, Zhonghai Yang, Fei Gao, Lixia Chen, Gary Guishan Xiao, J. Molnár, Qi Wang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aim: To investigate whether down-regulation of P-glycoprotein (P-gp) is correlated to resistance to cisplatin and VP-16 in four histopathological subtype cell lines of lung cancer (SK-MES-1, SPCA-1, NCI-H-460 and NCI-H-446). Materials and Methods: After pretreatment with or without verapamil, the P-gp expression was examined by means of RT-PCR and immunofluorescence. Cell survival on treatment with cisplatin and VP-16 was determined by MTT assay. Results: The expression of P-gp was clearly inhibited by verapamil in all four cell lines. Following pretreatment with verapamil, NCI-H-446 was more sensitive to cisplatin, while SPCA-1, NCI-H-460 and NCI-H-446 were more sensitive to VP-16 compared to the control. Conclusion: Down-regulation of P-gp is associated with intrinsic resistance to cisplatin in the NCI-H-446 and to VP-16 in SPCA-1, NCI-H-460 and NCI-H-446 cell lines. These findings indicate that down-regulation of P-gp may be helpful for the reversion of drug resistance in some lung cancer cell line subtypes.

Original languageEnglish
Pages (from-to)3593-3598
Number of pages6
JournalAnticancer Research
Volume30
Issue number9
Publication statusPublished - 2010

Fingerprint

P-Glycoprotein
Etoposide
Cisplatin
Lung Neoplasms
Down-Regulation
Cell Line
Verapamil
Drug Resistance
Fluorescent Antibody Technique
Cell Survival
Polymerase Chain Reaction

Keywords

  • Down-regulation
  • Drug resistance
  • Lung cancer
  • P-glycoprotein

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Wang, J., Wang, H., Zhao, L., Fan, S., Yang, Z., Gao, F., ... Wang, Q. (2010). Down-regulation of P-glycoprotein is associated with resistance to cisplatin and VP-16 in human lung cancer cell lines. Anticancer Research, 30(9), 3593-3598.

Down-regulation of P-glycoprotein is associated with resistance to cisplatin and VP-16 in human lung cancer cell lines. / Wang, Jiarui; Wang, Huiling; Zhao, Long; Fan, Sufang; Yang, Zhonghai; Gao, Fei; Chen, Lixia; Xiao, Gary Guishan; Molnár, J.; Wang, Qi.

In: Anticancer Research, Vol. 30, No. 9, 2010, p. 3593-3598.

Research output: Contribution to journalArticle

Wang, J, Wang, H, Zhao, L, Fan, S, Yang, Z, Gao, F, Chen, L, Xiao, GG, Molnár, J & Wang, Q 2010, 'Down-regulation of P-glycoprotein is associated with resistance to cisplatin and VP-16 in human lung cancer cell lines', Anticancer Research, vol. 30, no. 9, pp. 3593-3598.
Wang, Jiarui ; Wang, Huiling ; Zhao, Long ; Fan, Sufang ; Yang, Zhonghai ; Gao, Fei ; Chen, Lixia ; Xiao, Gary Guishan ; Molnár, J. ; Wang, Qi. / Down-regulation of P-glycoprotein is associated with resistance to cisplatin and VP-16 in human lung cancer cell lines. In: Anticancer Research. 2010 ; Vol. 30, No. 9. pp. 3593-3598.
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AU - Xiao, Gary Guishan

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