Down regulation of ethylene production in 'royal gala' apples

G. Hrazdina, E. Kiss, Z. Galli, C. Rosenfield, J. L. Norelli, H. S. Aldwinckle

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

Apples are an important agricultural commodity worldwide. Most apples are produced for the fresh market and have to be stored under controlled atmosphere (CA) conditions to avoid excessive softening. The softening of fruits is the result of structural changes in the cell walls. under the control of the ripening hormone ethylene. Ethylene is synthesized in plants from S-adenosyl-L-methionine by a short pathway that consists of two enzymes: 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-amino-cyclopropane-1- carboxylic acid oxidase (ACO). To interfere with ethylene synthesis in plants we have cloned two ACS genes from ripening 'McIntosh' apples. The gene having the closest similarity to the ripeningrelated ACS gene in other fruits was used to make an antisense construct. 'Royal Gala' plants were transformed with the antisense construct using an Agrobacteriummediated transformation system. Transgenic plants were propagated on antibioticcontaining agar, transferred to the greenhouse for conditioning, and later to the field. Transgenic 'Royal Gala' fruits that developed on these trees were evaluated for morphological characteristics, ethylene production, ACS activity, and ripening parameters. Data indicate down regulation of ethylene production and reduced softening were achieved in some transgenic lines. This indicates improved storability of the ethylene down- regulated transgenic fruit and suggests that structural integrity of this fruit may be maintained simply under refrigerated storage and controlled humidity.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages239-251
Number of pages13
Volume628
Publication statusPublished - Dec 12 2003

Publication series

NameActa Horticulturae
Volume628
ISSN (Print)05677572

Fingerprint

ethylene production
apples
ethylene
fruits
ripening
1-aminocyclopropane-1-carboxylic acid
genetically modified organisms
acids
S-adenosylmethionine
fresh market
genes
agricultural products
storage quality
transgenic plants
humidity
agar
hormones
cell walls
greenhouses
synthesis

Keywords

  • ACC synthase
  • Antisense gene construct
  • Fruit quality
  • Malus x domestica
  • MdACS2
  • Shelf life
  • Softening

ASJC Scopus subject areas

  • Horticulture

Cite this

Hrazdina, G., Kiss, E., Galli, Z., Rosenfield, C., Norelli, J. L., & Aldwinckle, H. S. (2003). Down regulation of ethylene production in 'royal gala' apples. In Acta Horticulturae (Vol. 628, pp. 239-251). (Acta Horticulturae; Vol. 628).

Down regulation of ethylene production in 'royal gala' apples. / Hrazdina, G.; Kiss, E.; Galli, Z.; Rosenfield, C.; Norelli, J. L.; Aldwinckle, H. S.

Acta Horticulturae. Vol. 628 2003. p. 239-251 (Acta Horticulturae; Vol. 628).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hrazdina, G, Kiss, E, Galli, Z, Rosenfield, C, Norelli, JL & Aldwinckle, HS 2003, Down regulation of ethylene production in 'royal gala' apples. in Acta Horticulturae. vol. 628, Acta Horticulturae, vol. 628, pp. 239-251.
Hrazdina G, Kiss E, Galli Z, Rosenfield C, Norelli JL, Aldwinckle HS. Down regulation of ethylene production in 'royal gala' apples. In Acta Horticulturae. Vol. 628. 2003. p. 239-251. (Acta Horticulturae).
Hrazdina, G. ; Kiss, E. ; Galli, Z. ; Rosenfield, C. ; Norelli, J. L. ; Aldwinckle, H. S. / Down regulation of ethylene production in 'royal gala' apples. Acta Horticulturae. Vol. 628 2003. pp. 239-251 (Acta Horticulturae).
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