Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines

G. Gyulai, J. A. Gémesné, Zs Sági, G. Venczel, P. Pintér, Z. Kristóf, O. Törjék, L. Heszky, S. Bottka, J. Kiss, L. Zatykó

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Anther derived spontaneous doubled haploid (DH-R1) plants were developed from an intercultivar F1 hybrid (Holland) of type pepper cv. (Capsicum annuum L. var. annuum, 2n = 24). Regenerants were grown in greenhouses for seed production to R2-generation (DH-R2) prior to flow cytometric analysis which revealed DH-R1 plants with haploid (n = 12); doubled haploid, DH, (2n = 24); tetraploid (2n = 48); and aneuploid genomes. Pollen grains of anther cultures showed a high decrease in viability during the first two weeks of incubation time. The DNA samples of the 47 DH-R2 individuals were bulked in nine groups and subjected to PCR analysis for estimating the level of homogeneity versus heterogeneity in comparison to the control of anther donor bulk of five plants of F1 hybrid pepper cv. (DH-R0). In total 26 primers were applied, 22 of them revealed scorable PCR profiles with 54 scorable PCR bands. Of these, 19 (35.2%) bands generated by 7 primers were polymorphic. All of the nine bulks of DH-R2 plants were discriminated at least by one primer.

Original languageEnglish
Pages (from-to)168-174
Number of pages7
JournalJournal of Plant Physiology
Volume156
Issue number2
Publication statusPublished - 2000

Fingerprint

paprika
Capsicum
Haploidy
doubled haploids
Capsicum annuum
Polymerase Chain Reaction
pepper
anthers
seed productivity
Tetraploidy
anther culture
aneuploidy
Aneuploidy
Pollen
haploidy
Netherlands
tetraploidy
Seeds
viability
Genome

Keywords

  • Androgenesis
  • Anther culture
  • Doubled haploid
  • ISSR
  • Polymerase chain reaction
  • RAPD
  • SSR

ASJC Scopus subject areas

  • Plant Science

Cite this

Gyulai, G., Gémesné, J. A., Sági, Z., Venczel, G., Pintér, P., Kristóf, Z., ... Zatykó, L. (2000). Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. Journal of Plant Physiology, 156(2), 168-174.

Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. / Gyulai, G.; Gémesné, J. A.; Sági, Zs; Venczel, G.; Pintér, P.; Kristóf, Z.; Törjék, O.; Heszky, L.; Bottka, S.; Kiss, J.; Zatykó, L.

In: Journal of Plant Physiology, Vol. 156, No. 2, 2000, p. 168-174.

Research output: Contribution to journalArticle

Gyulai, G, Gémesné, JA, Sági, Z, Venczel, G, Pintér, P, Kristóf, Z, Törjék, O, Heszky, L, Bottka, S, Kiss, J & Zatykó, L 2000, 'Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines', Journal of Plant Physiology, vol. 156, no. 2, pp. 168-174.
Gyulai G, Gémesné JA, Sági Z, Venczel G, Pintér P, Kristóf Z et al. Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. Journal of Plant Physiology. 2000;156(2):168-174.
Gyulai, G. ; Gémesné, J. A. ; Sági, Zs ; Venczel, G. ; Pintér, P. ; Kristóf, Z. ; Törjék, O. ; Heszky, L. ; Bottka, S. ; Kiss, J. ; Zatykó, L. / Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. In: Journal of Plant Physiology. 2000 ; Vol. 156, No. 2. pp. 168-174.
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