Double-stranded RNA mycoviruses in Fusarium culmorum and Fusarium graminearum isolates

Beáta Tóth, Péter Fónad, Ákos Mesterházy, J. Varga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The presence of double-stranded RNA (dsRNA) elements in Fusarium culmorum and F. graminearum isolates of different origin, and the possible effect of these elements on pathogenicity and toxin production of the isolates were examined. Altogether 40 F. culmorum and 38 F. graminearum isolates were involved in this study, together with F. cerealis and F. pseudograminearum strains. Double-stranded RNA elements indicative of mycovirus infection were detected for the first time in 5 F. culmorum isolates. The isolates originated from the United States, The Netherlands, Australia and Israel. The dsRNA nature of the fragments was proved by RNase, DNase and S1 nuclease treatments. The sizes of the dsRNA elements varied between 0.6-3.95 kbp. A dsRNA element of 3.0 kbp in size was also detected in a F. graminearum isolate came from South Africa. None of the Central-European isolates examined were found to carry such elements. We also examined the mycotoxin producing abilities and pathogenicity of the dsRNA infected isolates. Four of the mycovirus infected F. culmorum isolates produced deoxynivalenol and zearalenone (chemotype I), while one of the isolates came from the USA produced nivalenol and fusarenone X (chemotype II). The dsRNA-containing F. graminearum isolate produced deoxynivalenol and zearalenone. In general, dsRNA-containing isolates were found to be as pathogenic to two wheat cultivars as dsRNA free isolates. RAPD analysis and sequence analysis of a putative reductase gene fragment indicated that dsRNA-containing isolates are scattered among dsRNA free isolates. Further work is in progress in our laboratory to characterize these dsRNA elements by sequence analysis.

Original languageEnglish
Pages (from-to)733-740
Number of pages8
JournalCereal Research Communications
Volume33
Issue number4
Publication statusPublished - 2005

Fingerprint

mycoviruses
Fusarium culmorum
Double-Stranded RNA
Fusarium graminearum
double-stranded RNA
Fusarium
Zearalenone
chemotypes
zearalenone
deoxynivalenol
Virulence
Fungal Viruses
pathogenicity
sequence analysis
RNA Sequence Analysis
nivalenol
deoxyribonucleases
Deoxyribonucleases
nucleases
Mycotoxins

Keywords

  • dsRNA
  • Fusarium head blight
  • Mycotoxins
  • Pathogenicity
  • Section Discolor

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Double-stranded RNA mycoviruses in Fusarium culmorum and Fusarium graminearum isolates. / Tóth, Beáta; Fónad, Péter; Mesterházy, Ákos; Varga, J.

In: Cereal Research Communications, Vol. 33, No. 4, 2005, p. 733-740.

Research output: Contribution to journalArticle

Tóth, Beáta ; Fónad, Péter ; Mesterházy, Ákos ; Varga, J. / Double-stranded RNA mycoviruses in Fusarium culmorum and Fusarium graminearum isolates. In: Cereal Research Communications. 2005 ; Vol. 33, No. 4. pp. 733-740.
@article{260d0638cc7f42c7977725e1fb453149,
title = "Double-stranded RNA mycoviruses in Fusarium culmorum and Fusarium graminearum isolates",
abstract = "The presence of double-stranded RNA (dsRNA) elements in Fusarium culmorum and F. graminearum isolates of different origin, and the possible effect of these elements on pathogenicity and toxin production of the isolates were examined. Altogether 40 F. culmorum and 38 F. graminearum isolates were involved in this study, together with F. cerealis and F. pseudograminearum strains. Double-stranded RNA elements indicative of mycovirus infection were detected for the first time in 5 F. culmorum isolates. The isolates originated from the United States, The Netherlands, Australia and Israel. The dsRNA nature of the fragments was proved by RNase, DNase and S1 nuclease treatments. The sizes of the dsRNA elements varied between 0.6-3.95 kbp. A dsRNA element of 3.0 kbp in size was also detected in a F. graminearum isolate came from South Africa. None of the Central-European isolates examined were found to carry such elements. We also examined the mycotoxin producing abilities and pathogenicity of the dsRNA infected isolates. Four of the mycovirus infected F. culmorum isolates produced deoxynivalenol and zearalenone (chemotype I), while one of the isolates came from the USA produced nivalenol and fusarenone X (chemotype II). The dsRNA-containing F. graminearum isolate produced deoxynivalenol and zearalenone. In general, dsRNA-containing isolates were found to be as pathogenic to two wheat cultivars as dsRNA free isolates. RAPD analysis and sequence analysis of a putative reductase gene fragment indicated that dsRNA-containing isolates are scattered among dsRNA free isolates. Further work is in progress in our laboratory to characterize these dsRNA elements by sequence analysis.",
keywords = "dsRNA, Fusarium head blight, Mycotoxins, Pathogenicity, Section Discolor",
author = "Be{\'a}ta T{\'o}th and P{\'e}ter F{\'o}nad and {\'A}kos Mesterh{\'a}zy and J. Varga",
year = "2005",
language = "English",
volume = "33",
pages = "733--740",
journal = "Cereal Research Communications",
issn = "0133-3720",
publisher = "Cereal Research Non-Profit Company",
number = "4",

}

TY - JOUR

T1 - Double-stranded RNA mycoviruses in Fusarium culmorum and Fusarium graminearum isolates

AU - Tóth, Beáta

AU - Fónad, Péter

AU - Mesterházy, Ákos

AU - Varga, J.

PY - 2005

Y1 - 2005

N2 - The presence of double-stranded RNA (dsRNA) elements in Fusarium culmorum and F. graminearum isolates of different origin, and the possible effect of these elements on pathogenicity and toxin production of the isolates were examined. Altogether 40 F. culmorum and 38 F. graminearum isolates were involved in this study, together with F. cerealis and F. pseudograminearum strains. Double-stranded RNA elements indicative of mycovirus infection were detected for the first time in 5 F. culmorum isolates. The isolates originated from the United States, The Netherlands, Australia and Israel. The dsRNA nature of the fragments was proved by RNase, DNase and S1 nuclease treatments. The sizes of the dsRNA elements varied between 0.6-3.95 kbp. A dsRNA element of 3.0 kbp in size was also detected in a F. graminearum isolate came from South Africa. None of the Central-European isolates examined were found to carry such elements. We also examined the mycotoxin producing abilities and pathogenicity of the dsRNA infected isolates. Four of the mycovirus infected F. culmorum isolates produced deoxynivalenol and zearalenone (chemotype I), while one of the isolates came from the USA produced nivalenol and fusarenone X (chemotype II). The dsRNA-containing F. graminearum isolate produced deoxynivalenol and zearalenone. In general, dsRNA-containing isolates were found to be as pathogenic to two wheat cultivars as dsRNA free isolates. RAPD analysis and sequence analysis of a putative reductase gene fragment indicated that dsRNA-containing isolates are scattered among dsRNA free isolates. Further work is in progress in our laboratory to characterize these dsRNA elements by sequence analysis.

AB - The presence of double-stranded RNA (dsRNA) elements in Fusarium culmorum and F. graminearum isolates of different origin, and the possible effect of these elements on pathogenicity and toxin production of the isolates were examined. Altogether 40 F. culmorum and 38 F. graminearum isolates were involved in this study, together with F. cerealis and F. pseudograminearum strains. Double-stranded RNA elements indicative of mycovirus infection were detected for the first time in 5 F. culmorum isolates. The isolates originated from the United States, The Netherlands, Australia and Israel. The dsRNA nature of the fragments was proved by RNase, DNase and S1 nuclease treatments. The sizes of the dsRNA elements varied between 0.6-3.95 kbp. A dsRNA element of 3.0 kbp in size was also detected in a F. graminearum isolate came from South Africa. None of the Central-European isolates examined were found to carry such elements. We also examined the mycotoxin producing abilities and pathogenicity of the dsRNA infected isolates. Four of the mycovirus infected F. culmorum isolates produced deoxynivalenol and zearalenone (chemotype I), while one of the isolates came from the USA produced nivalenol and fusarenone X (chemotype II). The dsRNA-containing F. graminearum isolate produced deoxynivalenol and zearalenone. In general, dsRNA-containing isolates were found to be as pathogenic to two wheat cultivars as dsRNA free isolates. RAPD analysis and sequence analysis of a putative reductase gene fragment indicated that dsRNA-containing isolates are scattered among dsRNA free isolates. Further work is in progress in our laboratory to characterize these dsRNA elements by sequence analysis.

KW - dsRNA

KW - Fusarium head blight

KW - Mycotoxins

KW - Pathogenicity

KW - Section Discolor

UR - http://www.scopus.com/inward/record.url?scp=28944441156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28944441156&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:28944441156

VL - 33

SP - 733

EP - 740

JO - Cereal Research Communications

JF - Cereal Research Communications

SN - 0133-3720

IS - 4

ER -