Double-stranded RNA elements and virus-like particles in Aspergilli

J. Varga, B. Tóth, S. Szencz, J. Molnár, C. Fekete, L. Szabó

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mycoviruses with double-stranded RNA (dsRNA) genomes are frequently encountered in Aspergillus isolates. A detailed study of such dsRNA elements in black Aspergillus isolates collected worldwide was carried out, and the data were analysed. The results indicate that about 10% of black Aspergilli are infected. However, the geographic distribution of infected isolates exhibits large variations; 3-13% of the isolates collected from different continents were found to carry dsRNA elements. Hybridization experiments indicated that electrophoretic banding patterns are not reliable tools for estimating the diversity of these mycovirus genomes. Among strains representing other Aspergillus sections, dsRNA segments indicative of mycovirus infection were observed for the first time in 4 species (A. leporis, A. petrakii, A. fumigatus and A. primulinus). The latter species is able to reproduce sexually. This is the second report on the detection of naturally-occurring dsRNAs in sexually reproducing Aspergillus species. The presence of virus-like particles in these and other Aspergilli was also examined by electron microscopy. Most infected Aspergillus isolates examined were found to carry virus-like particles in the size range 36-40 nm.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalActa Biologica Hungarica
Volume52
Issue number2-3
DOIs
Publication statusPublished - 2001

Fingerprint

virus particle
virus-like particles
Double-Stranded RNA
Aspergillus
RNA Viruses
double-stranded RNA
Viruses
Virion
RNA
genome
electron microscopy
range size
mycoviruses
Genes
Genome
Aspergillus fumigatus
Particle Size
Electron microscopy
particle size
Electron Microscopy

Keywords

  • Aspergillus
  • Double-stranded RNA
  • Mycovirus
  • Virus-like particle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Double-stranded RNA elements and virus-like particles in Aspergilli. / Varga, J.; Tóth, B.; Szencz, S.; Molnár, J.; Fekete, C.; Szabó, L.

In: Acta Biologica Hungarica, Vol. 52, No. 2-3, 2001, p. 355-363.

Research output: Contribution to journalArticle

Varga, J. ; Tóth, B. ; Szencz, S. ; Molnár, J. ; Fekete, C. ; Szabó, L. / Double-stranded RNA elements and virus-like particles in Aspergilli. In: Acta Biologica Hungarica. 2001 ; Vol. 52, No. 2-3. pp. 355-363.
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