Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane

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Abstract

A series of dansylated sterically hindered amines designed to trapping reactive oxygen species, were synthesized. Compounds were tested in isolated thylakoid membranes subjected to photoinhibition by excess photosynthetically active radiation (400-700 nm). DanePy showed good selectivity for singlet oxygen and the formation of nitroxide was detected by appearance of ESR signal and quenching fluorescence.

Original languageEnglish
Pages (from-to)649-652
Number of pages4
JournalFree Radical Biology and Medicine
Volume24
Issue number4
DOIs
Publication statusPublished - Mar 1 1998

Fingerprint

Singlet Oxygen
Thylakoids
Amines
Paramagnetic resonance
Quenching
Reactive Oxygen Species
Fluorescence
Radiation
Membranes
Sensors
DanePy

Keywords

  • ESR spectroscopy
  • Fluorescence
  • Free radical
  • Photoinhibition
  • Singlet oxygen

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

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KW - Singlet oxygen

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