Doppa induces cell death but not differentiation of U937 cells

Evidence for the involvement of PKC-βl in the regulation of apoptosis

J. Pongrácz, Elizabeth M. Deacon, Gerald D. Johnson, David Burnett, Janet M. Lord

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent reports have claimed that activation of protein kinase C (PKC)-β is sufficient for both differentiation and apoptosis in promyeloid HL60 cells. Phorbol esters which differentially activate PKC isoenzymes in vitro were used to induce differentiation and apoptosis in U937 cells; TPA and Dopp activate all U937 PKC isoenzymes, except PKC-ζ and Doppa activate only PKC-βl. At concentrations of Doppa below 50 nM, only PKC-βl was activated by 2 min and apoptosis was induced, but there was no differentiation of cells towards monocytes. TPA (1-25 nM) and Dopp (5-100 nM) activated PKC-α,-βl and-δ within 2 min and induced differentiation, but only increased apoptosis at the highest concentrations used. Thus, initial activation of PKC-βl is insufficient for differentiation of U937 cells, but may lead to the induction of apoptosis.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalLeukemia Research
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 1996

Fingerprint

U937 Cells
Protein Kinase C
Cell Death
Apoptosis
Isoenzymes
HL-60 Cells
Phorbol Esters
Monocytes
Cell Differentiation

Keywords

  • Apoptosis
  • Differentiation
  • Doppa
  • PKC translocation
  • Protein kinase C (PKC)
  • U937

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Doppa induces cell death but not differentiation of U937 cells : Evidence for the involvement of PKC-βl in the regulation of apoptosis. / Pongrácz, J.; Deacon, Elizabeth M.; Johnson, Gerald D.; Burnett, David; Lord, Janet M.

In: Leukemia Research, Vol. 20, No. 4, 04.1996, p. 319-326.

Research output: Contribution to journalArticle

Pongrácz, J. ; Deacon, Elizabeth M. ; Johnson, Gerald D. ; Burnett, David ; Lord, Janet M. / Doppa induces cell death but not differentiation of U937 cells : Evidence for the involvement of PKC-βl in the regulation of apoptosis. In: Leukemia Research. 1996 ; Vol. 20, No. 4. pp. 319-326.
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