Dopamine-immunoreactive neurones in the central nervous system of the pond snail Lymnaea stagnalis

K. Elekes, G. Kemenes, L. Hiripi, M. Geffard, P. R. Benjamin

Research output: Contribution to journalArticle

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Abstract

The distribution of dopamine and dopamine-immunoreactive neurones was studied in the central nervous system of the snail Lymnaea stagnalis. The results from immunocytochemical labelling were compared with those from the application of the glyoxylic acid fluorescence method and 6-hydroxydopamine-induced pigment labelling. Comparisons were also made between the number of dopamine immunoreactive neurones and the dopamine content of the ganglia, measured by high-performance liquid chromatography. Dopamine immunocytochemistry proved to be superior to the other two histochemical techniques in terms of specificity and sensitivity. The 6-hydroxydopamine-induced pigment labelling failed to prove a useful tool for the in vivo identification of all dopamine-containing neurones. The distribution and number of dopamine-immunoreactive neurones and levels of biochemically measured dopamine in specific ganglia showed a close correspondence. By using the results of the dopamine immunocytochemistry and glyoxylic acid technique, a detailed map of dopamine-containing neurones was constructed. Dopamine-containing inter- and intra-ganglionic axon tracts were also demonstrated. The mapping of dopamine-containing neurones will facilitate further neurophysiological analysis of dopaminergic neural mechanisms in Lymnaea.

Original languageEnglish
Pages (from-to)214-224
Number of pages11
JournalJournal of Comparative Neurology
Volume307
Issue number2
Publication statusPublished - 1991

Fingerprint

Lymnaea
Dopaminergic Neurons
Snails
Dopamine
Central Nervous System
Oxidopamine
Ganglia
Immunohistochemistry
Axons
Fluorescence
High Pressure Liquid Chromatography
Sensitivity and Specificity

Keywords

  • glyoxylic acid
  • HPLC
  • immunocytochemistry
  • mapping
  • pigment labelling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dopamine-immunoreactive neurones in the central nervous system of the pond snail Lymnaea stagnalis. / Elekes, K.; Kemenes, G.; Hiripi, L.; Geffard, M.; Benjamin, P. R.

In: Journal of Comparative Neurology, Vol. 307, No. 2, 1991, p. 214-224.

Research output: Contribution to journalArticle

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