Dopamine, as well as norepinephrine, is a link between noradrenergic nerve terminals and splenocytes

Agnes Bencsics, Henry Sershen, M. Baranyi, Audrey Hashim, Abel Lajtha, E. Vízi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effect of supramaximal electric field stimulation on 3H released from rat spleen strips was studied after loading with either [3H]dopamine ([3H]DA) or [3H]norepinephrine ([3H]NE). In some experiments, [3H]DA and [3H]NE stored in the tissue or released in response to electrical stimulation were separated from their tritiated metabolites using HPLC followed by radiochemical detection. The stimulations-evoked release of 3H after loading with either derivative was subject to negative feedback modulation through α2-adrenergic, D2-dopamine and muscarinic acetylcholine receptors, and could be prevented by either calcium removal or tetrodotoxin blocking of Na+ influx, indicating its neuronal and vesicular origin. After the separation of radioactive metabolites by HPLC, both the tissue loaded with [3H]DA and the fractions collected during electrical stimulation contained a considerable amount of [3H]NE, providing evidence that the neurons it originated from were adrenergic in function. [3H]DA was also released during electrical stimulation. Since the spleen does not receive dopaminergic innervation, it was concluded that the noradrenergic axon terminals in the spleen were able to take up DA, convert it in part into NE, and release it as both DA and NE in response to neural activity. The ratio of [3H]DA and [3H]NE in the spleen loaded with [3H]DA was found to be dependent on both temperature and time of loading, and could be modulated by various drugs such as desmethylimipramine, a NE uptake blocker, and disulfiram or fusaric acid, dopamine β-hydroxylase inhibitors. The phenomenon may reveal a new mechanism by which immunocytes in the spleen can be regulated by the neuroendocrine system.

Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalBrain Research
Volume761
Issue number2
DOIs
Publication statusPublished - Jul 4 1997

Fingerprint

Dopamine
Norepinephrine
Spleen
Electric Stimulation
Adrenergic Agents
Fusaric Acid
High Pressure Liquid Chromatography
Disulfiram
Neurosecretory Systems
Desipramine
Tetrodotoxin
Presynaptic Terminals
Muscarinic Receptors
Mixed Function Oxygenases
Calcium
Neurons
Temperature
Pharmaceutical Preparations

Keywords

  • Dopamine
  • Dopamine β-hydroxylase
  • Neuroimmunomodulation
  • Spleen
  • Stress
  • Sympathetic neurotransmitter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dopamine, as well as norepinephrine, is a link between noradrenergic nerve terminals and splenocytes. / Bencsics, Agnes; Sershen, Henry; Baranyi, M.; Hashim, Audrey; Lajtha, Abel; Vízi, E.

In: Brain Research, Vol. 761, No. 2, 04.07.1997, p. 236-243.

Research output: Contribution to journalArticle

Bencsics, Agnes ; Sershen, Henry ; Baranyi, M. ; Hashim, Audrey ; Lajtha, Abel ; Vízi, E. / Dopamine, as well as norepinephrine, is a link between noradrenergic nerve terminals and splenocytes. In: Brain Research. 1997 ; Vol. 761, No. 2. pp. 236-243.
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