The authors examined 3 English bulldog puppies died on 7-9th day with clinical signs of half-a-day-, one-day-apathy and respiratory distress and finally opistotonus, which puppies originated from a breed of 30 adult dogs, were born with caesarean section, developed properly and seemed to be healthy. In the other litters of the same age or which were born later in the breed, no similar disease was observed. At necropsy, interstitial pneumonia with oedema, accumulation of straw-yellow coagulable serum in the thoracic cavity, hepatitis with necroses of pinhead size and pinprick or lentil sized haemorrhages under the epicardium, serous membrane of the stomach and intestines and renal capsule were found. At pathohistological examination, intralobular-interstitial pneumonia with the development of necrotic foci and catarrhal hepatitis, acute inflammation of the spleen and intestines and lymphocytic encephalitis were found. Acidophylic inclusion bodies were mainly found in the nuclei of damaged hepatic cells. By electron microscope, herpesvirus-particles were demonstrated in the latters. Canine herpesvirus (CHV) was isolated from spleen, liver and kidney samples from 3 died puppies, on canine kidney cell culture in a thermostat of 5% CO2 content, 100% vapour content and 33°C. Using the DNA obtained from tissues of infected organs (liver, lung), presence of canine herpesvirus (CHV) was proven by molecular biological methods. During amplification TK gene characteristic to herpesvirus was detected by specific primer designed for the gene. Diagnosis was confirmed by physical mapping of amplified amplicon.
|Translated title of the contribution||Domestic diagnosis of death in puppies, caused by herpesvirus|
|Number of pages||10|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Jun 1 2004|
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